Publication: An Electrochemical Impedance Spectroscopy-Based Aptasensor for the Determination of SARS-CoV-2-RBD Using a Carbon Nanofiber-Gold Nanocomposite Modified Screen-Printed Electrode
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2022-03
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Tutors
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MDPI AG
Abstract
Worldwide, human health is affected by severe acute respiratory syndrome coronavirus
2 (SARS-CoV-2). Hence, the fabrication of the biosensors to diagnose SARS-CoV-2 is critical. In
this paper, we report an electrochemical impedance spectroscopy (EIS)-based aptasensor for the
determination of the SARS-CoV-2 receptor-binding domain (SARS-CoV-2-RBD). For this purpose, the
carbon nanofibers (CNFs) were first decorated with gold nanoparticles (AuNPs). Then, the surface of
the carbon-based screen-printed electrode (CSPE) was modified with the CNF–AuNP nanocomposite
(CSPE/CNF–AuNP). After that, the thiol-terminal aptamer probe was immobilized on the surface of
the CSPE/CNF–AuNP. The surface coverage of the aptamer was calculated to be 52.8 pmolcm-2.
The CSPE/CNF–AuNP/Aptamer was then used for the measurement of SARS-CoV-2-RBD by using
the EIS method. The obtained results indicate that the signal had a linear–logarithmic relationship in
the range of 0.01–64 nM with a limit of detection of 7.0 pM. The proposed aptasensor had a good
selectivity to SARS-CoV-2-RBD in the presence of human serum albumin; human immunoglobulins
G, A, and M, hemagglutinin, and neuraminidase. The analytical performance of the aptasensor
was studied in human saliva samples. The present study indicates a practical application of the
CSPE/CNF-AuNP/Aptamer for the determination of SARS-CoV-2-RBD in human saliva samples
with high sensitivity and accuracy.
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Keywords
Electrochemical impedance spectroscopy, Aptasensor, Sars-cov-2-rbd, CNF–auNP
Bibliographic citation
Amouzadeh Tabrizi, M., & Acedo, P. (2022). An Electrochemical Impedance Spectroscopy-Based Aptasensor for the Determination of SARS-CoV-2-RBD Using a Carbon Nanofiber–Gold Nanocomposite Modified Screen-Printed Electrode. In Biosensors, 12(3), 142-156