RT Journal Article
T1 An Electrochemical Impedance Spectroscopy-Based Aptasensor for the Determination of SARS-CoV-2-RBD Using a Carbon Nanofiber-Gold Nanocomposite Modified Screen-Printed Electrode
A1 Amouzadeh Tabrizi, Mahmoud
A1 Acedo Gallardo, Pablo
AB Worldwide, human health is affected by severe acute respiratory syndrome coronavirus2 (SARS-CoV-2). Hence, the fabrication of the biosensors to diagnose SARS-CoV-2 is critical. Inthis paper, we report an electrochemical impedance spectroscopy (EIS)-based aptasensor for thedetermination of the SARS-CoV-2 receptor-binding domain (SARS-CoV-2-RBD). For this purpose, thecarbon nanofibers (CNFs) were first decorated with gold nanoparticles (AuNPs). Then, the surface ofthe carbon-based screen-printed electrode (CSPE) was modified with the CNF–AuNP nanocomposite(CSPE/CNF–AuNP). After that, the thiol-terminal aptamer probe was immobilized on the surface ofthe CSPE/CNF–AuNP. The surface coverage of the aptamer was calculated to be 52.8 pmolcm-2.The CSPE/CNF–AuNP/Aptamer was then used for the measurement of SARS-CoV-2-RBD by usingthe EIS method. The obtained results indicate that the signal had a linear–logarithmic relationship inthe range of 0.01–64 nM with a limit of detection of 7.0 pM. The proposed aptasensor had a goodselectivity to SARS-CoV-2-RBD in the presence of human serum albumin; human immunoglobulinsG, A, and M, hemagglutinin, and neuraminidase. The analytical performance of the aptasensorwas studied in human saliva samples. The present study indicates a practical application of theCSPE/CNF-AuNP/Aptamer for the determination of SARS-CoV-2-RBD in human saliva sampleswith high sensitivity and accuracy.
PB MDPI AG
SN 2079-6374
YR 2022
FD 2022-03
LK https://hdl.handle.net/10016/35222
UL https://hdl.handle.net/10016/35222
LA eng
NO This project received funding from the European Union’s Horizon 2020 research and innovationprogram under the Marie Sklodowska-Curie grant agreement No 801538 and the Ministeriode Ciencia e Innovación/Agencia Estatal de Investigación por parte del Ministerio de Ciencia eInnovación, MCIN /AEI /10.13039/501100011033 (Spain), Project PID2020-116439GB-I00 and theComunidad de Madrid in the framework of the action. “Excelencia para el Profesorado Universitario”inside the V Plan Regional de Investigación Científica e Innovación Tecnológica 2016–2020.
DS e-Archivo
RD 27 jul. 2024