Publication: An Electrochemical Impedance Spectroscopy-Based Aptasensor for the Determination of SARS-CoV-2-RBD Using a Carbon Nanofiber-Gold Nanocomposite Modified Screen-Printed Electrode
dc.affiliation.dpto | UC3M. Departamento de Tecnología Electrónica | es |
dc.affiliation.grupoinv | UC3M. Grupo de Investigación: Sensores y Técnicas de Instrumentación | es |
dc.contributor.author | Amouzadeh Tabrizi, Mahmoud | |
dc.contributor.author | Acedo Gallardo, Pablo | |
dc.contributor.funder | Comunidad de Madrid | es |
dc.contributor.funder | European Commission | es |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | es |
dc.date.accessioned | 2022-06-22T08:58:22Z | |
dc.date.available | 2022-06-22T08:58:22Z | |
dc.date.issued | 2022-03 | |
dc.description.abstract | Worldwide, human health is affected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Hence, the fabrication of the biosensors to diagnose SARS-CoV-2 is critical. In this paper, we report an electrochemical impedance spectroscopy (EIS)-based aptasensor for the determination of the SARS-CoV-2 receptor-binding domain (SARS-CoV-2-RBD). For this purpose, the carbon nanofibers (CNFs) were first decorated with gold nanoparticles (AuNPs). Then, the surface of the carbon-based screen-printed electrode (CSPE) was modified with the CNF–AuNP nanocomposite (CSPE/CNF–AuNP). After that, the thiol-terminal aptamer probe was immobilized on the surface of the CSPE/CNF–AuNP. The surface coverage of the aptamer was calculated to be 52.8 pmolcm-2. The CSPE/CNF–AuNP/Aptamer was then used for the measurement of SARS-CoV-2-RBD by using the EIS method. The obtained results indicate that the signal had a linear–logarithmic relationship in the range of 0.01–64 nM with a limit of detection of 7.0 pM. The proposed aptasensor had a good selectivity to SARS-CoV-2-RBD in the presence of human serum albumin; human immunoglobulins G, A, and M, hemagglutinin, and neuraminidase. The analytical performance of the aptasensor was studied in human saliva samples. The present study indicates a practical application of the CSPE/CNF-AuNP/Aptamer for the determination of SARS-CoV-2-RBD in human saliva samples with high sensitivity and accuracy. | en |
dc.description.sponsorship | This project received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 801538 and the Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación por parte del Ministerio de Ciencia e Innovación, MCIN /AEI /10.13039/501100011033 (Spain), Project PID2020-116439GB-I00 and the Comunidad de Madrid in the framework of the action. “Excelencia para el Profesorado Universitario” inside the V Plan Regional de Investigación Científica e Innovación Tecnológica 2016–2020. | en |
dc.format.extent | 14 | |
dc.identifier.bibliographicCitation | Amouzadeh Tabrizi, M., & Acedo, P. (2022). An Electrochemical Impedance Spectroscopy-Based Aptasensor for the Determination of SARS-CoV-2-RBD Using a Carbon Nanofiber–Gold Nanocomposite Modified Screen-Printed Electrode. In Biosensors, 12(3), 142-156 | en |
dc.identifier.doi | https://doi.org/10.3390/bios12030142 | |
dc.identifier.issn | 2079-6374 | |
dc.identifier.publicationfirstpage | 142 | |
dc.identifier.publicationissue | 3 | |
dc.identifier.publicationlastpage | 156 | |
dc.identifier.publicationtitle | Biosensors | en |
dc.identifier.publicationvolume | 12 | |
dc.identifier.uri | https://hdl.handle.net/10016/35222 | |
dc.identifier.uxxi | AR/0000030919 | |
dc.language.iso | eng | en |
dc.publisher | MDPI AG | en |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/COFUND-GA-2017-801538 | en |
dc.relation.projectID | Gobierno de España. PID2020-116439GB-I00 | es |
dc.rights | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. | en |
dc.rights | Atribución 3.0 España | * |
dc.rights.accessRights | open access | en |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject.eciencia | Electrónica | es |
dc.subject.other | Electrochemical impedance spectroscopy | en |
dc.subject.other | Aptasensor | en |
dc.subject.other | Sars-cov-2-rbd | en |
dc.subject.other | CNF–auNP | en |
dc.title | An Electrochemical Impedance Spectroscopy-Based Aptasensor for the Determination of SARS-CoV-2-RBD Using a Carbon Nanofiber-Gold Nanocomposite Modified Screen-Printed Electrode | en |
dc.type | research article | * |
dc.type.hasVersion | VoR | * |
dspace.entity.type | Publication |
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