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An Electrochemical Impedance Spectroscopy-Based Aptasensor for the Determination of SARS-CoV-2-RBD Using a Carbon Nanofiber-Gold Nanocomposite Modified Screen-Printed Electrode

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dc.affiliation.dptoUC3M. Departamento de Tecnología Electrónicaes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: Sensores y Técnicas de Instrumentaciónes
dc.contributor.authorAmouzadeh Tabrizi, Mahmoud
dc.contributor.authorAcedo Gallardo, Pablo
dc.contributor.funderComunidad de Madrides
dc.contributor.funderEuropean Commissiones
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)es
dc.date.accessioned2022-06-22T08:58:22Z
dc.date.available2022-06-22T08:58:22Z
dc.date.issued2022-03
dc.description.abstractWorldwide, human health is affected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Hence, the fabrication of the biosensors to diagnose SARS-CoV-2 is critical. In this paper, we report an electrochemical impedance spectroscopy (EIS)-based aptasensor for the determination of the SARS-CoV-2 receptor-binding domain (SARS-CoV-2-RBD). For this purpose, the carbon nanofibers (CNFs) were first decorated with gold nanoparticles (AuNPs). Then, the surface of the carbon-based screen-printed electrode (CSPE) was modified with the CNF–AuNP nanocomposite (CSPE/CNF–AuNP). After that, the thiol-terminal aptamer probe was immobilized on the surface of the CSPE/CNF–AuNP. The surface coverage of the aptamer was calculated to be 52.8 pmolcm-2. The CSPE/CNF–AuNP/Aptamer was then used for the measurement of SARS-CoV-2-RBD by using the EIS method. The obtained results indicate that the signal had a linear–logarithmic relationship in the range of 0.01–64 nM with a limit of detection of 7.0 pM. The proposed aptasensor had a good selectivity to SARS-CoV-2-RBD in the presence of human serum albumin; human immunoglobulins G, A, and M, hemagglutinin, and neuraminidase. The analytical performance of the aptasensor was studied in human saliva samples. The present study indicates a practical application of the CSPE/CNF-AuNP/Aptamer for the determination of SARS-CoV-2-RBD in human saliva samples with high sensitivity and accuracy.en
dc.description.sponsorshipThis project received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 801538 and the Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación por parte del Ministerio de Ciencia e Innovación, MCIN /AEI /10.13039/501100011033 (Spain), Project PID2020-116439GB-I00 and the Comunidad de Madrid in the framework of the action. “Excelencia para el Profesorado Universitario” inside the V Plan Regional de Investigación Científica e Innovación Tecnológica 2016–2020.en
dc.format.extent14
dc.identifier.bibliographicCitationAmouzadeh Tabrizi, M., & Acedo, P. (2022). An Electrochemical Impedance Spectroscopy-Based Aptasensor for the Determination of SARS-CoV-2-RBD Using a Carbon Nanofiber–Gold Nanocomposite Modified Screen-Printed Electrode. In Biosensors, 12(3), 142-156en
dc.identifier.doihttps://doi.org/10.3390/bios12030142
dc.identifier.issn2079-6374
dc.identifier.publicationfirstpage142
dc.identifier.publicationissue3
dc.identifier.publicationlastpage156
dc.identifier.publicationtitleBiosensorsen
dc.identifier.publicationvolume12
dc.identifier.urihttps://hdl.handle.net/10016/35222
dc.identifier.uxxiAR/0000030919
dc.language.isoengen
dc.publisherMDPI AGen
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/COFUND-GA-2017-801538en
dc.relation.projectIDGobierno de España. PID2020-116439GB-I00es
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland.en
dc.rightsAtribución 3.0 España*
dc.rights.accessRightsopen accessen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subject.ecienciaElectrónicaes
dc.subject.otherElectrochemical impedance spectroscopyen
dc.subject.otherAptasensoren
dc.subject.otherSars-cov-2-rbden
dc.subject.otherCNF–auNPen
dc.titleAn Electrochemical Impedance Spectroscopy-Based Aptasensor for the Determination of SARS-CoV-2-RBD Using a Carbon Nanofiber-Gold Nanocomposite Modified Screen-Printed Electrodeen
dc.typeresearch article*
dc.type.hasVersionVoR*
dspace.entity.typePublication
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