Characterization of postnatal microglia as a model for in vitro microglial studies

Gómez Cruz, Clara

Publication:
Characterization of postnatal microglia as a model for in vitro microglial studies

dc.contributor.advisor	Desco Menéndez, Manuel
dc.contributor.advisor	Cano López, Eva
dc.contributor.author	Gómez Cruz, Clara
dc.contributor.departamento	UC3M. Departamento de Bioingeniería e Ingeniería Aeroespacial	es
dc.date.accessioned	2020-03-05T09:23:11Z
dc.date.available	2020-03-05T09:23:11Z
dc.date.issued	2019-07
dc.date.submitted	2019-07-05
dc.description.abstract	Microglia are the resident immune cells of the central nervous system. They have been found to play a major role in the development of different neurodegenerative diseases. In the case of Alzheimer’s disease, understanding microglial interactions with Aβ peptides, a major hallmark of the disease, could unveil potential therapeutic targets. In order to study this interaction, it is important to be able to replicate it in vitro. However, there is no current model for microglial cells in vitro. Primary cultures of rodent postnatal microglia are currently the most used in vitro model for microglial cells, as they are the simpler method to obtain large numbers of primary cells. Nevertheless, this study shows that postnatal microglia grown in vitro with granulocyte-macrophage colony-stimulating factor (GM-CSF) do not present the levels of expression of microglial signature markers (TMEM119, FCRLS, P2RY12, TREM2) found in adult microglia in vivo. This microglial signature was also downregulated in postnatal microglia compared to adult microglia. From immunostaining of tissue slices at postnatal day 3 (P3) it was shown that at least one of this characteristic microglial markers (P2RY12) was present in most of the neonatal microglial cells, although it had lower levels of expression than in adult cells. Together, these results show that postnatal microglia have an immature phenotype and that current culture conditions are not able to promote differentiation of these immature cells into an adult phenotype. For this reason, neonatal microglia are currently not a good model for microglial studies concerning diseases that affect the adult brain.	es
dc.description.degree	Ingeniería Biomédica	es
dc.identifier.uri	http://hdl.handle.net/10016/29838
dc.language.iso	eng	es
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 España	*
dc.rights.accessRights	open access	es
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/es/	*
dc.subject.eciencia	Biología y Biomedicina	es
dc.subject.other	Microglia	es
dc.subject.other	Immune cells	es
dc.subject.other	Central nervous system	es
dc.subject.other	In vitro	es
dc.title	Characterization of postnatal microglia as a model for in vitro microglial studies	es
dc.type	bachelor thesis	*
dspace.entity.type	Publication