Publication: Diseño y construcción de un sensor de permeabilidad de oxígeno en membranas mediante fluorescencia
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2010
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2010
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Abstract
En este Proyecto Fin de Carrera se presenta un estudio del estado de la técnica de sensores de oxígeno. En este estudio se analizan los sensores basados en la fluorescencia como los últimos y más modernos métodos para la medida de concentraciones de oxígeno, resultando estos una alternativa a los tradicionales sensores electroquímicos debido a sus mejores prestaciones y características. Además se aborda el diseño de un sistema de medida de permeabilidad y el desarrollo de un sensor para la determinación de la permeabilidad de oxígeno en membranas planas, basado en el quenching de la fluorescencia. La fabricación de este sistema incluye el análisis, diseño y construcción de una cámara de medida para la colocación en su interior de la membrana y una guía de luz de fibra óptica conectada a un fluorímetro. Así mismo se analiza el problema de la selección y fabricación del filme en el cual se incorpora la sonda fluorescente. Finalmente se realizaron las comprobaciones necesarias para evaluar el funcionamiento del sistema de medida e introducir las mejoras necesarias para su optimización. _______________________________________________________
On this Final Degree Project it is presented a survey of the state of the technique of the oxygen sensors. This project is focused on the sensors based on the fluorescence techniques, being those the latest and most modern methods for the measure of oxygen concentration, being those an alternative of the traditional electrochemical sensors due to their better characteristics. In addition it is also shown the design of a permeability measure system and the development of a sensor for the determination of the permeability of the oxygen in flat membranes, based on the fluoresced quenching. The manufacture of this system also includes the analysis, design and fabrication of a measure chamber for the placement inside of both the membrane and an optic fiber light guide connected to a fluorometer. Furthermore it is also analyzed the issue of selecting and manufacturing a film to which a fluorescent probe is added. Finally the proper functionality of the system is checked and new proposals for the system optimization are proposed.
On this Final Degree Project it is presented a survey of the state of the technique of the oxygen sensors. This project is focused on the sensors based on the fluorescence techniques, being those the latest and most modern methods for the measure of oxygen concentration, being those an alternative of the traditional electrochemical sensors due to their better characteristics. In addition it is also shown the design of a permeability measure system and the development of a sensor for the determination of the permeability of the oxygen in flat membranes, based on the fluoresced quenching. The manufacture of this system also includes the analysis, design and fabrication of a measure chamber for the placement inside of both the membrane and an optic fiber light guide connected to a fluorometer. Furthermore it is also analyzed the issue of selecting and manufacturing a film to which a fluorescent probe is added. Finally the proper functionality of the system is checked and new proposals for the system optimization are proposed.
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Keywords
Sensores de oxígeno, Membranas (Tecnología), Fluorescencia, Permeabilidad