Quantitative performance characterization of three-dimensional noncontact fluorescence molecular tomography

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dc.contributor.author Favicchio, Rosy
dc.contributor.author Psycharakis, Stylianos
dc.contributor.author Schönig, Kai
dc.contributor.author Bartsch, Dusan
dc.contributor.author Mamalaki, Clio
dc.contributor.author Papamatheakis, Joseph
dc.contributor.author Ripoll Lorenzo, Jorge
dc.contributor.author Zacharakis, Giannis
dc.date.accessioned 2016-12-15T11:46:02Z
dc.date.available 2016-12-15T11:46:02Z
dc.date.issued 2016-02-01
dc.identifier.bibliographicCitation The Journal of Biomedical Optics, 2016, 21(2), p. 026009.
dc.identifier.issn 1083-3668
dc.identifier.uri http://hdl.handle.net/10016/23953
dc.description.abstract Fluorescent proteins and dyes are routine tools for biological research to describe the behavior of genes, proteins, and cells, as well as more complex physiological dynamics such as vessel permeability and pharmacokinetics. The use of these probes in whole body in vivo imaging would allow extending the range and scope of current biomedical applications and would be of great interest. In order to comply with a wide variety of application demands, in vivo imaging platform requirements span from wide spectral coverage to precise quantification capabilities. Fluorescence molecular tomography (FMT) detects and reconstructs in three dimensions the distribution of a fluorophore in vivo. Noncontact FMT allows fast scanning of an excitation source and noninvasive measurement of emitted fluorescent light using a virtual array detector operating in free space. Here, a rigorous process is defined that fully characterizes the performance of a custom-built horizontal noncontact FMT setup. Dynamic range, sensitivity, and quantitative accuracy across the visible spectrum were evaluated using fluorophores with emissions between 520 and 660 nm. These results demonstrate that high-performance quantitative three-dimensional visible light FMT allowed the detection of challenging mesenteric lymph nodes in vivo and the comparison of spectrally distinct fluorescent reporters in cell culture. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher SPIE
dc.rights © The Autors, 2016
dc.rights Atribución-NoComercial-SinDerivadas 3.0 España
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.other molecular imaging
dc.subject.other fluorescence molecular tomography
dc.subject.other quantification
dc.subject.other fluorescent probes
dc.subject.other diffuse optical tomography
dc.subject.other in-vivo
dc.subject.other scattering media
dc.subject.other protein
dc.subject.other mice
dc.subject.other differentiation
dc.subject.other reconstruction
dc.subject.other approximation
dc.subject.other validation
dc.subject.other expression
dc.title Quantitative performance characterization of three-dimensional noncontact fluorescence molecular tomography
dc.type article
dc.relation.publisherversion https://doi.org/10.1117/1.JBO.21.2.026009
dc.subject.eciencia Biología y Biomedicina
dc.identifier.doi 10.1117/1.JBO.21.2.026009
dc.rights.accessRights openAccess
dc.type.version acceptedVersion
dc.identifier.publicationissue 2
dc.identifier.publicationtitle Journal of Biomedical Optics
dc.identifier.publicationvolume 21
dc.identifier.uxxi AR/0000017822
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