Publication: Implementación de un laboratorio de microscopía confocal de materiales
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Publication date
2014-06
Defense date
2014-06-25
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Abstract
La microscopía confocal es una técnica basada en la observación de un único plano
focal, con un aporte mínimo de señal por el resto de los planos iluminados. Nació a
medidados del siglo XX, pero hasta muy recientemente no ha encontrado su lugar como
técnica de observación y medición en otros campos, distintos de los que le venían siendo
habituales (biomédicos).
En 2013 se adquiere e implementa un sistema de microscopía confocal, formado por
dos equipos de Leica (DCM 3D y TCS-SP8) en el Centro Tecnológico de Repsol, el cual
no sólo permitirá observar y medir con precisión observables a los que antes era difícil
acceder, sino que permitirá realizar nuevas tareas y descargar de trabajo algunas técnicas
que tradicionalmente venían estando saturadas, como por ejemplo, el microscopio
electrónico de barrido.
Con este proyecto, se pretende describir el proceso de implementación de los
equipos, así como ilustrar para qué usarlos y qué puede esperarse de ellos en cuanto a
aplicaciones. También se describirá todo el trabajo realizado para la puesta a punto de
métodos e informes con los que cubrir diferentes necesidades del Centro Tecnológico de
Repsol.
Confocal microscopy is a technique based on the observation of a single focal plane with a minimal input signal from the rest of the enlightened planes. Created in the mind- 20th century, it was not until recent years when this technique found its place as an observation and measurement technique in fields, others than those in which its use was widely spread (biomedical). In 2013, the Repsol Technology Centre acquires and implements a confocal microscopy system, consisting of two Leica’s hardware (DCM 3D and TCS-SP8). This iniciative will not only allow to observe and measure with great accuracy, observables whose examination were difficult with previous approches, but also to perform new tasks and relieve some techniques traditionally saturated, as for example, the scanning electron microscope. This project aims to describe the implementation process of the equipment as well as to illustrate how to use them and what can be expected of them in terms of applications. All the work done for the development of methods and reports that cover different Repsol Technology Centre needs are also described.
Confocal microscopy is a technique based on the observation of a single focal plane with a minimal input signal from the rest of the enlightened planes. Created in the mind- 20th century, it was not until recent years when this technique found its place as an observation and measurement technique in fields, others than those in which its use was widely spread (biomedical). In 2013, the Repsol Technology Centre acquires and implements a confocal microscopy system, consisting of two Leica’s hardware (DCM 3D and TCS-SP8). This iniciative will not only allow to observe and measure with great accuracy, observables whose examination were difficult with previous approches, but also to perform new tasks and relieve some techniques traditionally saturated, as for example, the scanning electron microscope. This project aims to describe the implementation process of the equipment as well as to illustrate how to use them and what can be expected of them in terms of applications. All the work done for the development of methods and reports that cover different Repsol Technology Centre needs are also described.
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Keywords
Microscopía confocal, Centro Tecnológico de Repsol, Óptica, Instrumentación