Publication: Plenoptic projection fluorescence tomography
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2014-09-16
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Optical Society of America (OSA)
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Abstract
A new method to obtain the three-dimensional localization of fluorochrome distributions in micrometric samples is presented. It uses a microlens array coupled to the image port of a standard microscope to obtain tomographic data by a filtered back-projection algorithm. Scanning of the microlens array is proposed to obtain a dense data set for reconstruction. Simulation and experimental results are shown and the implications of this approach in fast 3D imaging are discussed.
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Keywords
Three-dimensional microscopy, Fluorescence microscopy, Tomographic image processing
Bibliographic citation
Optics express: the international online journal of optics, vol. 22 issue 19 (Sept., 2014)