Evaluation of autofocus functions in molecular cytogenetic analysis

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dc.contributor.author Santos, Andrés
dc.contributor.author Ortiz de Solórzano, C.
dc.contributor.author Vaquero López, Juan José
dc.contributor.author Peña, J. M.
dc.contributor.author Malpica, Norberto
dc.contributor.author Pozo, Fernando del
dc.date.accessioned 2012-06-12T11:40:17Z
dc.date.available 2012-06-12T11:40:17Z
dc.date.issued 1997-12
dc.identifier.bibliographicCitation Journal of Microscopy, vol. 188, n. 3, dic. 1997. Pp. 264-272
dc.identifier.issn 0022-2720 (print version)
dc.identifier.issn 1365-2818 (online version)
dc.identifier.uri http://hdl.handle.net/10016/14539
dc.description.abstract This work describes a systematic evaluation of several autofocus functions used for analytical fluorescent image cytometry studies of counterstained nuclei. Focusing is the first step in the automatic fluorescence in situ hybridization analysis of cells. Thirteen functions have been evaluated using qualitative and quantitative procedures. For the last of these procedures a figure-of-merit (FOM) is defined and proposed. This new FOM takes into account five important features of the focusing function. Our results show that functions based on correlation measures have the best performance for this type of image.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Wiley
dc.rights Atribución-NoComercial-SinDerivadas 3.0 España
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.other Analytical cytometry
dc.subject.other Autofocus
dc.subject.other FISH
dc.subject.other Focusing algorithms
dc.title Evaluation of autofocus functions in molecular cytogenetic analysis
dc.type article
dc.description.status Publicado
dc.relation.publisherversion http://dx.doir.org/10.1046/j.1365-2818.1997.2630819.x
dc.subject.eciencia Biología y Biomedicina
dc.identifier.doi 10.1046/j.1365-2818.1997.2630819.x
dc.rights.accessRights openAccess
dc.type.version acceptedVersion
dc.identifier.publicationfirstpage 264
dc.identifier.publicationissue n. 3
dc.identifier.publicationlastpage 272
dc.identifier.publicationtitle Journal of Microscopy
dc.identifier.publicationvolume vol. 188
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