Publication: Evaluation of autofocus functions in molecular cytogenetic analysis
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Identifiers
Publication date
1997-12
Defense date
Advisors
Tutors
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract
This work describes a systematic evaluation of several
autofocus functions used for analytical fluorescent image
cytometry studies of counterstained nuclei. Focusing is the
first step in the automatic fluorescence in situ hybridization
analysis of cells. Thirteen functions have been evaluated
using qualitative and quantitative procedures. For the last
of these procedures a figure-of-merit (FOM) is defined and
proposed. This new FOM takes into account five important
features of the focusing function. Our results show that
functions based on correlation measures have the best
performance for this type of image.
Description
Keywords
Analytical cytometry, Autofocus, FISH, Focusing algorithms
Bibliographic citation
Journal of Microscopy, vol. 188, n. 3, dic. 1997. Pp. 264-272