Publication:
Covalently Labeled Fluorescent Exosomes for In Vitro and In Vivo Applications

Simple item page
dc.affiliation.dptoUC3M. Departamento de Bioingenieríaes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: Biomedical Imaging and Instrumentation Groupes
dc.contributor.authorGonzález, María Isabel
dc.contributor.authorGonzález Arjona, Mario
dc.contributor.authorSantos Coquillat, Ana
dc.contributor.authorVaquero, Javier
dc.contributor.authorVázquez Ogando, Elena
dc.contributor.authorMolina, Antonio de
dc.contributor.authorPeinado, Héctor
dc.contributor.authorDesco Menéndez, Manuel
dc.contributor.authorSalinas Rodríguez, Beatriz
dc.contributor.funderComunidad de Madrides
dc.date.accessioned2021-09-07T09:48:08Z
dc.date.available2021-09-07T09:48:08Z
dc.date.issued2021-01
dc.descriptionThis article belongs to the Special Issue Optical Nanoparticles for Biomedicineen
dc.description.abstractThe vertiginous increase in the use of extracellular vesicles and especially exosomes for therapeutic applications highlights the necessity of advanced techniques for gaining a deeper knowledge of their pharmacological properties. Herein, we report a novel chemical approach for the robust attachment of commercial fluorescent dyes to the exosome surface with covalent binding. The applicability of the methodology was tested on milk and cancer cell-derived exosomes (from U87 and B16F10 cancer cells). We demonstrated that fluorescent labeling did not modify the original physicochemical properties of exosomes. We tested this nanoprobe in cell cultures and healthy mice to validate its use for in vitro and in vivo applications. We confirmed that these fluorescently labeled exosomes could be successfully visualized with optical imaging.en
dc.description.sponsorshipThis study was supported by the Comunidad de Madrid, projects: “Y2018/NMT-4949 (NanoLiver-CM)” and “S2017/BMD-3867 (RENIM-CM)”; it was also co-funded by the European Structural and Investment Fund. The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN), and the Pro CNIC Foundation, and it is a Severo Ochoa Center of Excellence (SEV-2015-0505). JV was supported by grants from Instituto de Salud Carlos III (PI18/01833), co-funded by European Regional Development Fund (ERDF) and from Comunidad de Madrid, project “S2017/BMD2737 (ExoHep-CM)”, co-funded by European Structural and Investment Fund. A. Santos-Coquillat is grateful for the financial support from Ministerio de Ciencia e Innovación, Instituto de Salud Carlos III Sara Borrell Fellowship grant CD19/00136.en
dc.identifier.bibliographicCitationGonzález, M. I., González-Arjona, M., Santos-Coquillat, A., Vaquero, J., Vázquez-Ogando, E., de Molina, A., Peinado, H., Desco, M. & Salinas, B. (2021). Covalently Labeled Fluorescent Exosomes for In Vitro and In Vivo Applications. Biomedicines, 9(1), 81.en
dc.identifier.doihttps://doi.org/10.3390/biomedicines9010081
dc.identifier.issn2227-9059
dc.identifier.publicationfirstpage1
dc.identifier.publicationissue1
dc.identifier.publicationlastpage16
dc.identifier.publicationtitleBiomedicinesen
dc.identifier.publicationvolume9
dc.identifier.urihttps://hdl.handle.net/10016/33241
dc.identifier.uxxiAR/0000028059
dc.language.isoengen
dc.relation.projectIDComunidad de Madrid. Y2018/NMT-4949es
dc.relation.projectIDComunidad de Madrid. S2017/BMD-3867es
dc.relation.projectIDComunidad de Madrid. S2017/BMD2737es
dc.rightsAtribución 3.0 España*
dc.rights© 2021 by the authors.en
dc.rights.accessRightsopen accessen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subject.ecienciaBiología y Biomedicinaes
dc.subject.otherExosomesen
dc.subject.otherExtracellular vesiclesen
dc.subject.otherFluorescenceen
dc.subject.otherOptical imagingen
dc.subject.otherVesicle labelingen
dc.titleCovalently Labeled Fluorescent Exosomes for In Vitro and In Vivo Applicationsen
dc.typeresearch article*
dc.type.hasVersionVoR*
dspace.entity.typePublication
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Covalently_Biomedicines_2021.pdf
Size:
2.29 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
DBIAB - BIIG - Journal Articles