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Photopatterned Antibodies for Selective Cell Attachment

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dc.affiliation.dptoUC3M. Departamento de Ciencia e Ingeniería de Materiales e Ingeniería Químicaes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: Polímeros y Compositeses
dc.contributor.authorde Almeida Custódio, Catarina
dc.contributor.authorSan Miguel Arnanz, Verónica
dc.contributor.authorGropeanu, Radu A.
dc.contributor.authorGropeanu, Mihaela
dc.contributor.authorWirkner, Melanie
dc.contributor.authorReis, Rui L.
dc.contributor.authorMano, João F.
dc.contributor.authordel Campo, Aránzazu
dc.date.accessioned2017-05-10T08:53:32Z
dc.date.available2017-05-10T08:53:32Z
dc.date.issued2014-08-26
dc.description.abstractWe present a phototriggerable system that allows for the spatiotemporal controlled attachment of selected cell types to a biomaterial using immobilized antibodies that specifically target individual cell phenotypes. o-Nitrobenzyl caged biotin was used to functionalize chitosan membranes and mediate site-specific coupling of streptavidin and biotinylated antibodies after light activation. The ability of this system to capture and immobilize specific cells on a surface was tested using endothelial-specific biotinylated antibodies and nonspecific ones as controls. Homogeneous patterned monolayers of human umbilical vein endothelial cells were obtained on CD31-functionalized surfaces. This is a simple and generic approach that is applicable to other ligands, materials, and cell types and shows the flexibility of caged ligands to trigger and control the interaction between cells and biomaterials.en
dc.description.sponsorshipWe thank Martina Knecht (MPIP) for help with the synthesis of caged biotin and Dr. Ron Unger and Prof. C. J. Kirkpatrick (University Clinic Mainz, RepairLab) for providing HUVECs. C.A.C. acknowledges funding support from the Portuguese Foundation for Science and Technology (FCT) (fellowship SFRH/BD/61390/2009) and from the International Max-Planck Research School in Mainz. The research leading to these results has received funding from the European Union’s Seventh Framework Programme (FP7/2007-2013) under grant agreement no. REGPOT-CT2012-316331-POLARIS.en
dc.description.sponsorshipEuropean Community's Seventh Framework Programen
dc.format.extent6
dc.format.mimetypeapplication/pdf
dc.identifier.bibliographicCitationLangmuir, 2014, 30 (33), pp. 10066-10071.
dc.identifier.doihttp://www.dx.doi.org/10.1021/la502688h
dc.identifier.issn0743-7463
dc.identifier.publicationfirstpage10066
dc.identifier.publicationissue33
dc.identifier.publicationlastpage10071
dc.identifier.publicationtitleLangmuiren
dc.identifier.publicationvolume30
dc.identifier.urihttps://hdl.handle.net/10016/24545
dc.identifier.uxxiAR/0000019823
dc.language.isoengen
dc.publisherAmerican Chemical Societyen
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/316331en
dc.rights© 2014 American Chemical Societyen
dc.rights.accessRightsopen access
dc.subject.ecienciaMaterialeses
dc.subject.ecienciaQuímicaes
dc.titlePhotopatterned Antibodies for Selective Cell Attachmenten
dc.typeresearch article*
dc.type.hasVersionAM*
dspace.entity.typePublication
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