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Novel LTCC-potentiometric microfluidic device for biparametric analysis of organic compounds carrying plastic antibodies as ionophores: Application to sulfamethoxazole and trimethoprim

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dc.affiliation.dptoUC3M. Departamento de Ciencia e Ingeniería de Materiales e Ingeniería Químicaes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: Síntesis y Procesado de Materialeses
dc.contributor.authorAlmeida, S.A.A.
dc.contributor.authorArasa, E.
dc.contributor.authorPuyol, M.
dc.contributor.authorMartínez Cisneros, Cynthia Susana
dc.contributor.authorMontenegro, M.C.B.S.M.
dc.contributor.authorSales, M.G.F.
dc.date.accessioned2020-11-06T12:35:58Z
dc.date.available2020-11-06T12:35:58Z
dc.date.issued2011-12-15
dc.description.abstractMonitoring organic environmental contaminants is of crucial importance to ensure public health. This requires simple, portable and robust devices to carry out on-site analysis. For this purpose, a low-temperature co-fired ceramics (LTCC) microfluidic potentiometric device (LTCC/ POT) was developed for the first time for an organic compound: sulfamethoxazole (SMX). Sensory materials relied on newly designed plastic antibodies. Sol–gel, self-assembling monolayer and molecular-imprinting techniques were merged for this purpose. Silica beads were amine-modified and linked to SMX via glutaraldehyde modification. Condensation polymerization was conducted around SMX to fill the vacant spaces. SMX was removed after, leaving behind imprinted sites of complementary shape. The obtained particles were used as ionophores in plasticized PVC membranes. The most suitable membrane composition was selected in steady-state assays. Its suitability to flow analysis was verified in flow-injection studies with regular tubular electrodes. The LTCC/ POT device integrated a bidimensional mixer, an embedded reference electrode based on Ag/AgCl and an Ag-based contact screen-printed under a micromachined cavity of 600 m depth. The sensing membranes were deposited over this contact and acted as indicating electrodes. Under optimum conditions, the SMX sensor displayed slopes of about −58.7 mV/decade in a range from 12.7 to 250 g/mL, providing a detection limit of 3.85 g/mL and a sampling throughput of 36 samples/h with a reagent consumption of 3.3 mL per sample. The system was adjusted later to multiple analyte detection by including a second potentiomet-ric cell on the LTCC/ POT device. No additional reference electrode was required. This concept was applied to Trimethoprim (TMP), always administered concomitantly with sulphonamide drugs, and tested in fish-farming waters. The biparametric microanalyzer displayed Nernstian behaviour, with aver-age slopes −54.7 (SMX) and +57.8 (TMP) mV/ decade. To demonstrate the microanalyzer capabilities for real applications, it was successfully applied to single and simultaneous determination of SMX and TMP in aquaculture waters.en
dc.description.sponsorshipThe authors acknowledge the financial support from FCT, Fundacão para a Ciência e Tecnologia/FEDER (project PTDC/AGR-AAM/68359/2006). Oneofus (Almeida SAA) is grateful to FCT for the PhD Grant (SFRH/BD/42509/2007).en
dc.description.statusPublicadoes
dc.format.extent6
dc.identifier.bibliographicCitationBiosensors and Bioelectronics, (2011), 30(1), pp.: 197-203.en
dc.identifier.doihttps://doi.org/10.1016/j.bios.2011.09.011
dc.identifier.issn0956-5663
dc.identifier.publicationfirstpage197
dc.identifier.publicationissue1
dc.identifier.publicationlastpage203
dc.identifier.publicationtitleBiosensors and Bioelectronicsen
dc.identifier.publicationvolume30
dc.identifier.urihttps://hdl.handle.net/10016/31340
dc.identifier.uxxiAR/0000026047
dc.language.isoengen
dc.publisherElsevieren
dc.rights© 2011 Elsevier B.V. All rights reserved.en
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.accessRightsopen accessen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subject.ecienciaMaterialeses
dc.subject.otherBiparametric analysisen
dc.subject.otherCeramic tapesen
dc.subject.otherMiniaturizationne
dc.subject.otherPotentiometryen
dc.subject.otherSulfamethoxazolene
dc.subject.otherTrimethoprimen
dc.titleNovel LTCC-potentiometric microfluidic device for biparametric analysis of organic compounds carrying plastic antibodies as ionophores: Application to sulfamethoxazole and trimethoprimen
dc.typeresearch article*
dc.type.hasVersionAM*
dspace.entity.typePublication
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