Publication:
Preparation and characterization of plasma-derived fibrin hydrogels modified by alginate di-aldehyde

Simple item page
dc.affiliation.dptoUC3M. Departamento de Tecnología Electrónicaes
dc.affiliation.dptoUC3M. Departamento de Bioingenieríaes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: Sensores y Técnicas de Instrumentaciónes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: Tissue Engineering and Regenerative Medicine (TERMeG)es
dc.contributor.authorSanz Horta, Raúl
dc.contributor.authorMatesanz Fernandez-Arias, Ana
dc.contributor.authorJorcano Noval, José Luis
dc.contributor.authorVelasco Bayón, Diego
dc.contributor.authorAcedo Gallardo, Pablo
dc.contributor.authorGallardo, Alberto
dc.contributor.authorReinecke, Helmut
dc.contributor.funderComunidad de Madrides
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es
dc.date.accessioned2023-01-31T08:51:07Z
dc.date.available2023-01-31T08:51:07Z
dc.date.issued2022-04-02
dc.descriptionThis article belongs to the Special Issue Biomaterials and Regenerative Medicine.en
dc.description.abstractFibrin hydrogels are one of the most popular scaffolds used in tissue engineering due to their excellent biological properties. Special attention should be paid to the use of human plasma-derived fibrin hydrogels as a 3D scaffold in the production of autologous skin grafts, skeletal muscle regeneration and bone tissue repair. However, mechanical weakness and rapid degradation, which causes plasma-derived fibrin matrices to shrink significantly, prompted us to improve their stability. In our study, plasma-derived fibrin was chemically bonded to oxidized alginate (alginate di-aldehyde, ADA) at 10%, 20%, 50% and 80% oxidation, by Schiff base formation, to produce natural hydrogels for tissue engineering applications. First, gelling time studies showed that the degree of ADA oxidation inhibits fibrin polymerization, which we associate with fiber increment and decreased fiber density; moreover, the storage modulus increased when increasing the final volume of CaCl2 (1% w/v) from 80 µL to 200 µL per milliliter of hydrogel. The contraction was similar in matrices with and without human primary fibroblasts (hFBs). In addition, proliferation studies with encapsulated hFBs showed an increment in cell viability in hydrogels with ADA at 10% oxidation at days 1 and 3 with 80 µL of CaCl2; by increasing this compound (CaCl2), the proliferation does not significantly increase until day 7. In the presence of 10% alginate oxidation, the proliferation results are similar to the control, in contrast to the sample with 20% oxidation whose proliferation decreases. Finally, the viability studies showed that the hFB morphology was maintained regardless of the degree of oxidation used; however, the quantity of CaCl2 influences the spread of the hFBsen
dc.description.sponsorshipThis research was supported by Programa de Actividades de I + D entre Grupos de Investigación de la Comunidad de Madrid, S2018/BAA-4480, Biopieltec-CM, Programa Estatal de I + D + i Orientada a los Retos de la Sociedad, RTI2018-101627-B-I00, and Cátedra Fundación Ramón Areces.en
dc.format.extent17
dc.identifier.bibliographicCitationSanz-Horta, R., Matesanz, A., Jorcano, J. L., Velasco, D., Acedo, P., Gallardo, A., Reinecke, H. & Elvira, C. (2022). Preparation and Characterization of Plasma-Derived Fibrin Hydrogels Modified by Alginate di-Aldehyde. International Journal of Molecular Sciences, 23(8), 4296.en
dc.identifier.doihttps://doi.org/10.3390/ijms23084296
dc.identifier.issn1422-0067
dc.identifier.publicationfirstpage1
dc.identifier.publicationissue8, 4296
dc.identifier.publicationlastpage17
dc.identifier.publicationtitleInternational Journal of Molecular Sciencesen
dc.identifier.publicationvolume23
dc.identifier.urihttps://hdl.handle.net/10016/36420
dc.identifier.uxxiAR/0000030463
dc.language.isoeng
dc.publisherMDPI
dc.relation.projectIDComunidad de Madrid. S2018/BAA-4480es
dc.relation.projectIDGobierno de España. RTI2018-101627-B-I00es
dc.rights© 2022 by the authors.en
dc.rightsAtribución 3.0 España*
dc.rights.accessRightsopen accessen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subject.ecienciaAeronáuticaes
dc.subject.ecienciaBiología y Biomedicinaes
dc.subject.ecienciaElectrónicaes
dc.subject.ecienciaTelecomunicacioneses
dc.subject.otherFibrin hydrogelsen
dc.subject.otherPlasma-derived fibrin hydrogelen
dc.subject.otherPlatelet-poor plasma (PPP)en
dc.subject.otherOxidized alginateen
dc.subject.otherAlginate di-aldehyde hydrogelsen
dc.subject.otherTissue engineeringen
dc.subject.otherSkin tissue engineeringen
dc.subject.otherSkeletal muscle regenerationen
dc.subject.otherBone tissue reparationen
dc.titlePreparation and characterization of plasma-derived fibrin hydrogels modified by alginate di-aldehydeen
dc.typeresearch article*
dc.type.hasVersionVoR*
dspace.entity.typePublication
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Preparation_IJMS_2022.pdf
Size:
4.23 MB
Format:
Adobe Portable Document Format
Download
Collections
DBIAB - TERMEG - Journal Articles
DTE - STI - Artículos de Revistas