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Evaluation of different methodologies for primary human dermal fibroblast spheroid formation: automation through 3D bioprinting technology

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dc.affiliation.dptoUC3M. Departamento de Mecánica de Medios Continuos y Teoría de Estructurases
dc.affiliation.dptoUC3M. Departamento de Bioingenieríaes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: Dinámica y Fractura de Elementos Estructuraleses
dc.affiliation.grupoinvUC3M. Grupo de Investigación: Tissue Engineering and Regenerative Medicine (TERMeG)es
dc.contributor.authorQuílez López, Cristina
dc.contributor.authorCerdeira Valtierra, Enrique
dc.contributor.authorGonzalez-Rico Iriarte, Jorge
dc.contributor.authorAranda Izuzquiza, Gonzalo De
dc.contributor.authorLópez-Donaire, María Luisa
dc.contributor.authorJorcano Noval, José Luis
dc.contributor.authorVelasco Bayón, Diego
dc.contributor.funderComunidad de Madrides
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es
dc.date.accessioned2023-02-06T12:22:32Z
dc.date.available2023-02-06T12:22:32Z
dc.date.issued2022-09
dc.description.abstractCell spheroids have recently emerged as an effective tool to recapitulate native microenvironments of living organisms in an in vitro scenario, increasing the reliability of the results obtained and broadening their applications in regenerative medicine, cancer research, disease modeling and drug screening. In this study the generation of spheroids containing primary human dermal fibroblasts was approached using the two-widely employed methods: hanging-drop and U-shape low adhesion plate (LA-plate). Moreover, extrusion-based three-dimensional (3D) bioprinting was introduced to achieve a standardized and scalable production of cell spheroids, decreasing considerably the possibilities of human error. This was ensured when U-shape LA-plates were used, showing an 85% formation efficiency, increasing up to a 98% when it was automatized using the 3D bioprinting technologies. However, sedimentation effect within the cartridge led to a reduction of 20% in size of the spheroid during the printing process. Hyaluronic acid (HA) was chosen as viscosity enhancer to supplement the bioink and overcome cell sedimentation within the cartridge due to the high viability values exhibited by the cells -around 80%- at the used conditions. Finally, (ANCOVA) of spheroid size over time for different printing conditions stand out HA 0.4% (w/v) 60 kDa as the viscosity-improved bioink that exhibit the highest cell viability and spheroid formation percentages. Besides, not only did it ensure cell spheroid homogeneity over time, reducing cell sedimentation effects, but also wider spheroid diameters over time with less variability, outperforming significantly manual loading.en
dc.description.sponsorshipWe kindly thank Daniel García for their guidance with the rheological experiments. This work was supported by Programa de Actividades de I + D entre Grupos de Investigación de la Comunidad de Madrid, S2018/ BAA-4480, Biopieltec-CM, Programa Estatal de I + D + i Orientada a los Retos de la Sociedad, RTI2018-101627-B-I00 and Cátedra Fundación Ramón Areces. The experimental techniques used during this study were performed in the CleanRooms of Bioengineering, Universidad Carlos III de Madrid, Madrid, Spain.en
dc.format.extent11
dc.identifier.bibliographicCitationQuílez, C., Cerdeira, E., González-Rico, J., de Aranda, G., López-Donaire, M. L., Jorcano, J. L. & Velasco, D. (2022). Evaluation of different methodologies for primary human dermal fibroblast spheroid formation: automation through 3D bioprinting technology. Biomedical Materials, 17(5), 055002.en
dc.identifier.doihttps://doi.org/10.1088/1748-605X/ac7a7f
dc.identifier.issn1748-6041
dc.identifier.publicationfirstpage1
dc.identifier.publicationissue5, 055002
dc.identifier.publicationlastpage11
dc.identifier.publicationtitleBiomedical Materialsen
dc.identifier.publicationvolume17
dc.identifier.urihttps://hdl.handle.net/10016/36479
dc.identifier.uxxiAR/0000030972
dc.language.isoeng
dc.publisherIOP Scienceen
dc.relation.projectIDComunidad de Madrid. S2018/BAA-4480es
dc.relation.projectIDGobierno de España. RTI2018-101627-B-I00es
dc.rights© 2022 The Author(s).en
dc.rightsAtribución 3.0 España*
dc.rights.accessRightsopen accessen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subject.ecienciaBiología y Biomedicinaes
dc.subject.ecienciaIngeniería Mecánicaes
dc.subject.otherCell spheroidsen
dc.subject.otherPrimary human dermal fibroblasts (DHFS)en
dc.subject.otherU-shape low adhesion plateen
dc.subject.other3D bioprintingen
dc.subject.otherViscosity enhanceren
dc.subject.otherHyaluronic aciden
dc.subject.otherGlycerolen
dc.titleEvaluation of different methodologies for primary human dermal fibroblast spheroid formation: automation through 3D bioprinting technologyes
dc.typeresearch article*
dc.type.hasVersionVoR*
dspace.entity.typePublication
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