Suppression of F-18-FDG signal in the bladder on small animal PET-CT

Cusso Mula, Lorena; Desco Menéndez, Manuel

Publication:
Suppression of F-18-FDG signal in the bladder on small animal PET-CT

dc.affiliation.dpto	UC3M. Departamento de Bioingeniería	es
dc.affiliation.grupoinv	UC3M. Grupo de Investigación: Biomedical Imaging and Instrumentation Group	es
dc.affiliation.grupoinv	UC3M. Grupo de Investigación: BSEL - Laboratorio de Ciencia e Ingeniería Biomédica	es
dc.contributor.author	Cusso Mula, Lorena
dc.contributor.author	Desco Menéndez, Manuel
dc.date.accessioned	2023-11-17T12:31:03Z
dc.date.available	2023-11-17T12:31:03Z
dc.date.issued	2018-10-17
dc.description.abstract	Introduction Retention of 2-deoxy-2-[F-18]fluoro-D-glucose F-18-FDG in the bladder causes more problems in small animal research than in human research owing to the smaller size of the subject. Catheterization has been proposed to reduce bladder spillover both in human studies and in small animal research. Noninvasive alternatives such as hydration plus furosemide also seem to be a promising pre-imaging strategy for decreasing bladder spillover. Our main goal was to measure the effects of the combination of furosemide and hydration for reducing bladder signal directly on mouse bowel F-18-FDG-PET images. Methods Nine mice were divided into two groups: the control group (C, n = 4) and the treatment group (n = 5). The clearance protocol combines hyperhydration and a single furosemide dose during the F-18-FDG uptake period. Two images were acquired on different days in treated mice to evaluate two different furosemide doses (low dose, LD, 3.5 mg/kg; and high dose, HD, 7 mg/kg). A region of interest was drawn on each computed tomography image (bladder, kidneys, liver, muscle, and bone marrow). To quantify bladder spillover, two different areas of the colon were selected. Results A remarkable reduction in bladder spillover was achieved on F-18-FDG-PET in both groups. Our imaging findings were quantified, and both furosemide doses induced a decrease in mean standard uptake values (SUVmean) compared with the controls (LD 1.46 +/- 0.54 and HD 1.05 +/- 0.29; controls: 8.90 +/- 3.4 [p-value < 0.05]). Conclusion We validated a non-invasive, easy, and harmless pre-imaging alternative for decreasing F-18-FDG bladder spillover. Our study shows the effect of furosemide on bladder spillover directly on F-18-FDG-PET images by measuring SUVmean in the bladder, colon, liver, muscle, and bone marrow.	en
dc.format.extent	9	es
dc.format.mimetype	application/pdf	en
dc.identifier.bibliographicCitation	Cussó, L., & Desco, M. (2018). Suppression of 18F-FDG signal in the bladder on small animal PET-CT. P. K. Garg (Ed.), PLOS ONE 13(10), p. e0205610.	en
dc.identifier.doi	10.1371/journal.pone.0205610
dc.identifier.issn	1932-6203
dc.identifier.publicationissue	10	es
dc.identifier.publicationtitle	PLoS One	en
dc.identifier.publicationvolume	13	es
dc.identifier.uri	https://hdl.handle.net/10016/38899
dc.identifier.uxxi	AR/0000022291
dc.language.iso	eng	en
dc.publisher	P. K. Garg	en
dc.rights	© 2018 Cussó, Desco.	en
dc.rights	Atribución 3.0 España	*
dc.rights.accessRights	open access	en
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/es/	*
dc.subject.eciencia	Biología y Biomedicina	es
dc.subject.other	Furosemide	en
dc.subject.other	Bladder	en
dc.subject.other	18f-Fdg-pet Images	en
dc.title	Suppression of F-18-FDG signal in the bladder on small animal PET-CT	en
dc.type	research article	*
dc.type.hasVersion	VoR	*
dspace.entity.type	Publication