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Isolation of goat milk small extracellular vesicles by novel combined bio-physical methodology

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dc.affiliation.dptoUC3M. Departamento de Bioingenieríaes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: Biomedical Imaging and Instrumentation Groupes
dc.contributor.authorGonzález Vasco, María Isabel
dc.contributor.authorGallardo Alguacil, Begoña
dc.contributor.authorCeron, Carlos
dc.contributor.authorAguilera-Jimenez, Elena
dc.contributor.authorCortes-Canteli, Marta
dc.contributor.authorPeinado, Hector
dc.contributor.authorDesco Menéndez, Manuel
dc.contributor.authorSalinas Rodríguez, Beatriz
dc.contributor.funderComunidad de Madrides
dc.contributor.funderMinisterio de Educación, Cultura y Deporte (España)es
dc.date.accessioned2024-02-01T09:27:30Z
dc.date.available2024-02-01T09:27:30Z
dc.date.issued2023-09-27
dc.description.abstractIntroduction: Goat milk is notable as a cost-effective source of exosomes, also known as small extracellular vesicles (sEVs). These nanoparticle-like structures are naturally secreted by cells and have emerged as potential diagnostic agents and drug delivery systems, also supported by their proven therapeutic effects. However, the complexity of goat milk and the lack of standardized protocols make it difficult to isolate pure sEVs. This work presents an optimized approach that combines well-established physical isolation methods with the biological treatment of milk with rennet.Methods: sEVs derived from goat milk were purified using a methodology that combines differential ultracentrifugation, rennet, and size-exclusion chromatography. This novel strategy was compared with two of the main methodologies developed for isolating extracellular vesicles from bovine and human milk by means of physico-chemical characterization of collected vesicles using Transmission Electron Microscopy, Western blot, Bradford Coomassie assay, Dynamic Light Scattering, Nanoparticle Tracking Analysis and Zeta Potential.Results: Vesicles isolated with the optimized protocol had sEV-like characteristics and high homogeneity, while samples obtained with the previous methods were highly aggregated, with significant residual protein content.Discussion: This work provides a novel biophysical methodology for isolating highly enriched goat milk sEVs samples with high stability and homogeneity, for their further evaluation in biomedical applications as diagnostic tools or drug delivery systems.en
dc.description.sponsorshipThis study has been funded by the Instituto de Salud Carlos III through the projects "PI20/01632" and "PT20/00044", and co-funded by European Regional Development Fund (ERDF, "A way to make Europe"), and the PRISMAP project. This work has been also funded by Comunidad de Madrid through the project "S2022/BMD-7403 RENIM-CM." The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovacion (MCIN), and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (CEX 2020-001041-S). MIG is funded by the Instituto de Investigacion Sanitaria Gregorio Maranon, Intramural Programme for the Promotion of R & D & I 2021, Sub-programme "Predoctoral training contract." MC-C is funded by a Miguel Servet type II research contract (CPII21/00007) from the Instituto de Salud Carlos III, Madrid, Spain. CC is funded by a predoctoral fellowship from Fundacion Espanola de Trombosis y Hemostasia (FETH-SETH).r This study has been funded by the Instituto de Salud Carlos III through the projects "PI20/01632" and "PT20/00044", and co-funded by European Regional Development Fund (ERDF, "A way to make Europe"), and the PRISMAP project. This work has been also funded by Comunidad de Madrid through the project "S2022/BMD-7403 RENIM-CM." The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovacion (MCIN), and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (CEX 2020-001041-S). MIG is funded by the Instituto de Investigacion Sanitaria Gregorio Maranon, Intramural Programme for the Promotion of R & D & I 2021, Sub-programme "Predoctoral training contract." MC-C is funded by a Miguel Servet type II research contract (CPII21/00007) from the Instituto de Salud Carlos III, Madrid, Spain. CC is funded by a predoctoral fellowship from Fundacion Espanola de Trombosis y Hemostasia (FETH-SETH).en
dc.format.extent8
dc.identifier.bibliographicCitationGonzález MI, Gallardo B, Cerón C, Aguilera-Jiménez E, Cortes-Canteli M, Peinado H, Desco M and Salinas B (2023) Isolation of goat milk small extracellular vesicles by novel combined bio-physical methodology. Front. Bioeng. Biotechnol. 11:1197780en
dc.identifier.doihttps://doi.org/10.3389/fbioe.2023.1197780
dc.identifier.issn2296-4185
dc.identifier.publicationfirstpage1
dc.identifier.publicationlastpage8
dc.identifier.publicationtitleFrontiers in Bioengineering and Biotechnologyen
dc.identifier.publicationvolume11, 1197780
dc.identifier.urihttps://hdl.handle.net/10016/39710
dc.identifier.uxxiAR/0000034066
dc.language.isoengen
dc.publisherFrontiersen
dc.relation.projectIDComunidad de Madrid. S2022/BMD-7403es
dc.rightsCopyright © 2023 González, Gallardo, Cerón, Aguilera-Jiménez, Cortes-Canteli, Peinado, Desco and Salinas.en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rightsAtribución 3.0 España*
dc.rights.accessRightsopen accesses
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subject.ecienciaBiología y Biomedicinaes
dc.subject.ecienciaMedicinaes
dc.subject.otherGoat milken
dc.subject.otherExosomesen
dc.subject.otherSmall extracellular vesiclesen
dc.subject.otherIsolation methodsen
dc.subject.otherDifferential ultracentrifugationen
dc.subject.otherExosome characterizationen
dc.titleIsolation of goat milk small extracellular vesicles by novel combined bio-physical methodologyen
dc.typeresearch articleen
dc.type.hasVersionVoRen
dspace.entity.typePublication
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