Publication: Influence of absorption and scattering on the quantification of fluorescence diffuse optical tomography using normalized data
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2012-03
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Society of Photo-optical Instrumentation Engineers
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Abstract
Reconstruction algorithms for imaging fluorescence in near infrared ranges usually normalize fluores
cence light with respect to excitation light. Using this approach, we investigated the influence of absorption
and scattering heterogeneities on quantification accuracy when assuming a homogeneous model and explored
possible reconstruction improvements by using a heterogeneous model. To do so, we created several compu
ter simulated phantoms: a homogeneous slab phantom (P1), slab phantoms including a region with a two to
six fold increase in scattering (P2) and in absorption (P3), and an atlas based mouse phantom that modeled different
liver and lung scattering (P4). For P1, reconstruction with the wrong optical properties yielded quantification errors
that increased almost linearly with the scattering coefficient while they were mostly negligible regarding the absorp
tion coefficient. This observation agreed with the theoretical results. Taking the quantification of a homogeneous
phantom as a reference, relative quantification errors obtained when wrongly assuming homogeneous media were
in the range þ41 to þ94% (P2), 0.1 to −7% (P3), and −39 to þ44% (P4). Using a heterogeneous model, the overall
error ranged from −7 to 7%. In conclusion, this work demonstrates that assuming homogeneous media leads to
noticeable quantification errors that can be improved by adopting heterogeneous models.
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Tomography, Diffusion, Fluorescence, Scattering
Bibliographic citation
Journal of Biomedical Optics, vol. 17, n. 3, mar. 2012, Pp. 1-10