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Influence of absorption and scattering on the quantification of fluorescence diffuse optical tomography using normalized data

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dc.affiliation.dptoUC3M. Departamento de Bioingenieríaes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: Biomedical Imaging and Instrumentation Groupes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: BSEL - Laboratorio de Ciencia e Ingeniería Biomédicaes
dc.contributor.authorPérez-Juste Abascal, Juan Felipe
dc.contributor.authorAguirre, Juan
dc.contributor.authorChamorro Servent, Judit
dc.contributor.authorSchweiger, Martin
dc.contributor.authorArridge, Simon
dc.contributor.authorRipoll Lorenzo, Jorge
dc.contributor.authorVaquero López, Juan José
dc.contributor.authorDesco Menéndez, Manuel
dc.date.accessioned2012-07-11T10:17:19Z
dc.date.available2012-07-11T10:17:19Z
dc.date.issued2012-03
dc.description.abstractReconstruction algorithms for imaging fluorescence in near infrared ranges usually normalize fluores cence light with respect to excitation light. Using this approach, we investigated the influence of absorption and scattering heterogeneities on quantification accuracy when assuming a homogeneous model and explored possible reconstruction improvements by using a heterogeneous model. To do so, we created several compu ter simulated phantoms: a homogeneous slab phantom (P1), slab phantoms including a region with a two to six fold increase in scattering (P2) and in absorption (P3), and an atlas based mouse phantom that modeled different liver and lung scattering (P4). For P1, reconstruction with the wrong optical properties yielded quantification errors that increased almost linearly with the scattering coefficient while they were mostly negligible regarding the absorp tion coefficient. This observation agreed with the theoretical results. Taking the quantification of a homogeneous phantom as a reference, relative quantification errors obtained when wrongly assuming homogeneous media were in the range þ41 to þ94% (P2), 0.1 to −7% (P3), and −39 to þ44% (P4). Using a heterogeneous model, the overall error ranged from −7 to 7%. In conclusion, this work demonstrates that assuming homogeneous media leads to noticeable quantification errors that can be improved by adopting heterogeneous models.en
dc.description.sponsorshipThis study was supported by Ministerio de Ciencia e Innovación(FPI program, TEC 2008 06715, and CENIT AMIT CEN 20101014), Comunidad de Madrid and European Regional Development Fund ARTEMIS S2009/DPI 1802, and EU FP7 project FMTXCT 201792.en
dc.description.statusPublicado
dc.format.mimetypeapplication/pdf
dc.identifier.bibliographicCitationJournal of Biomedical Optics, vol. 17, n. 3, mar. 2012, Pp. 1-10en
dc.identifier.doi10.1117/1.JBO.17.3.036013
dc.identifier.issn1083-3668 (print version)
dc.identifier.issn1560-2281 (online version)
dc.identifier.publicationfirstpage1
dc.identifier.publicationissue3
dc.identifier.publicationlastpage10
dc.identifier.publicationtitleJournal of Biomedical Optics
dc.identifier.publicationvolume17
dc.identifier.urihttps://hdl.handle.net/10016/14885
dc.identifier.uxxiAR/0000009989es
dc.language.isoeng
dc.publisherSociety of Photo-optical Instrumentation Engineersen
dc.relation.projectIDComunidad de Madrid. S2009/DPI-1802/ARTEMISes
dc.relation.projectIDGobierno de España. TEC2008-06715
dc.relation.publisherversionhttp://dx.doi.org/10.1117/1.JBO.17.3.036013
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.ecienciaBiología y Biomedicinaes
dc.subject.otherTomographyen
dc.subject.otherDiffusionen
dc.subject.otherFluorescenceen
dc.subject.otherScatteringen
dc.titleInfluence of absorption and scattering on the quantification of fluorescence diffuse optical tomography using normalized dataen
dc.typeresearch article*
dc.type.hasVersionVoR*
dspace.entity.typePublication
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