León, Patricia deCañas Arranz, RodrigoSáez Achaerandio, YagoForner, MarDefaus, SiraCuadra Fernández, María DoloresBustos, María J.Torres, ElisaAndreu, DavidBlanco, EstherSobrino, FranciscoHammer, Sabine E.2022-02-112022-02-112020-09-08de León, P.; Cañas-Arranz, R.; Saez, Y.; Forner, M.; Defaus, S.; Cuadra, D.; Bustos, M.J.; Torres, E.; Andreu, D.; Blanco, E.; Sobrino, F.; Hammer, S.E. Association of Porcine Swine Leukocyte Antigen (SLA) Haplotypes with B- and T-Cell Immune Response to Foot-and-Mouth Disease Virus (FMDV) Peptides. Vaccines 2020, 8, 513. https://doi.org/10.3390/vaccines80305132076-393Xhttps://hdl.handle.net/10016/34107Dendrimer peptides are promising vaccine candidates against the foot-and-mouth disease virus (FMDV). Several B-cell epitope (B2T) dendrimers, harboring a major FMDV antigenic B-cell site in VP1 protein, are covalently linked to heterotypic T-cell epitopes from 3A and/or 3D proteins, and elicited consistent levels of neutralizing antibodies and IFN-gamma-producing cells in pigs. To address the contribution of the highly polymorphic nature of the porcine MHC (SLA, swine leukocyte antigen) on the immunogenicity of B2T dendrimers, low-resolution (Lr) haplotyping was performed. We looked for possible correlations between particular Lr haplotypes with neutralizing antibody and T-cell responses induced by B2T peptides. In this study, 63 pigs immunized with B2T dendrimers and 10 non-immunized (control) animals are analyzed. The results reveal a robust significant correlation between SLA class-II Lr haplotypes and the T-cell response. Similar correlations of T-cell response with SLA class-I Lr haplotypes, and between B-cell antibody response and SLA class-I and SLA class-II Lr haplotypes, were only found when the sample was reduced to animals with Lr haplotypes represented more than once. These results support the contribution of SLA class-II restricted T-cells to the magnitude of the T-cell response and to the antibody response evoked by the B2T dendrimers, being of potential value for peptide vaccine design against FMDV.eng© 2020 by the authorsAtribución 3.0 Españaadaptive immunitydendrimer peptidefoot-and-mouth diseae virusprotectionsla polymorphismswineswine leukocyte antigenvaccineresearch articleInformáticahttps://doi.org/10.3390/vaccines8030513open access1316Vaccines8AR/0000027477