RT Journal Article
T1 Covalently Labeled Fluorescent Exosomes for In Vitro and In Vivo Applications
A1 González, María Isabel
A1 González Arjona, Mario
A1 Santos Coquillat, Ana
A1 Vaquero, Javier
A1 Vázquez Ogando, Elena
A1 Molina, Antonio de
A1 Peinado, Héctor
A1 Desco Menéndez, Manuel
A1 Salinas Rodríguez, Beatriz
AB The vertiginous increase in the use of extracellular vesicles and especially exosomes for therapeutic applications highlights the necessity of advanced techniques for gaining a deeper knowledge of their pharmacological properties. Herein, we report a novel chemical approach for the robust attachment of commercial fluorescent dyes to the exosome surface with covalent binding. The applicability of the methodology was tested on milk and cancer cell-derived exosomes (from U87 and B16F10 cancer cells). We demonstrated that fluorescent labeling did not modify the original physicochemical properties of exosomes. We tested this nanoprobe in cell cultures and healthy mice to validate its use for in vitro and in vivo applications. We confirmed that these fluorescently labeled exosomes could be successfully visualized with optical imaging.
SN 2227-9059
YR 2021
FD 2021-01
LK https://hdl.handle.net/10016/33241
UL https://hdl.handle.net/10016/33241
LA eng
NO This article belongs to the Special Issue Optical Nanoparticles for Biomedicine
NO This study was supported by the Comunidad de Madrid, projects: “Y2018/NMT-4949 (NanoLiver-CM)” and “S2017/BMD-3867 (RENIM-CM)”; it was also co-funded by the European Structural and Investment Fund. The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN), and the Pro CNIC Foundation, and it is a Severo Ochoa Center of Excellence (SEV-2015-0505). JV was supported by grants from Instituto de Salud Carlos III (PI18/01833), co-funded by European Regional Development Fund (ERDF) and from Comunidad de Madrid, project “S2017/BMD2737 (ExoHep-CM)”, co-funded by European Structural and Investment Fund. A. Santos-Coquillat is grateful for the financial support from Ministerio de Ciencia e Innovación, Instituto de Salud Carlos III Sara Borrell Fellowship grant CD19/00136.
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RD 1 jun. 2024