RT Conference Proceedings
T1 Fluorescence multi-scale endoscopy and its applications in the study and diagnosis of gastro-intestinal diseases: set-up design and software implementation
A1 Gómez García, Pablo Aurelio
A1 Arranz, Alicia
A1 Fresno, Manuel
A1 Desco Menéndez, Manuel
A1 Mahmood, Umar
A1 Vaquero López, Juan José
A1 Ripoll Lorenzo, Jorge
AB Endoscopy is frequently used in the diagnosis of several gastro-intestinal pathologies as Crohn disease, ulcerative colitis or colorectal cancer. It has great potential as a non-invasive screening technique capable of detecting suspicious alterations in the intestinal mucosa, such as inflammatory processes. However, these early lesions usually cannot be detected with conventional endoscopes, due to lack of cellular detail and the absence of specific markers. Due to this lack of specificity, the development of new endoscopy technologies, which are able to show microscopic changes in the mucosa structure, are necessary. We here present a confocal endomicroscope, which in combination with a wide field fluorescence endoscope offers fast and specific macroscopic information through the use of activatable probes and a detailed analysis at cellular level of the possible altered tissue areas. This multi-modal and multi-scale imaging module, compatible with commercial endoscopes, combines near-infrared fluorescence (NIRF) measurements (enabling specific imaging of markers of disease and prognosis) and confocal endomicroscopy making use of a fiber bundle, providing a cellular level resolution. The system will be used in animal models exhibiting gastro-intestinal diseases in order to analyze the use of potential diagnostic markers in colorectal cancer. In this work, we present in detail the set-up design and the software implementation in order to obtain simultaneous RGB/NIRF measurements and short confocal scanning times.
PB Spie- The International Society For Optical Engineering
SN 9781628416961
YR 2015
FD 2015
LK https://hdl.handle.net/10016/21187
UL https://hdl.handle.net/10016/21187
LA eng
NO Proceedings of: IPA 2015 / SPIE Biophotonics South America. Rio de Janeiro, Brazil, 22-26 May, 2015
NO The authors acknowledge support from EC FP7 IMI project PREDICT-TB, the EC FP7 CIG grant HIGH-THROUGHPUT TOMO, the Spanish MINECO project grant FIS2013-41802-R MESO-IMAGING, and TOPUS S2013/MIT-3024 project from the regional government of Madrid
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RD 30 jun. 2024