RT Journal Article
T1 Association of porcine swine leukocyte antigen (Sla) haplotypes with b-and t-cell immune response to foot-and-mouth disease virus (fmdv) peptides
A1 León, Patricia de
A1 Cañas Arranz, Rodrigo
A1 Sáez Achaerandio, Yago
A1 Forner, Mar
A1 Defaus, Sira
A1 Cuadra Fernández, María Dolores
A1 Bustos, María J.
A1 Torres, Elisa
A1 Andreu, David
A1 Blanco, Esther
A1 Sobrino, Francisco
A1 Hammer, Sabine E.
AB Dendrimer peptides are promising vaccine candidates against the foot-and-mouth disease virus (FMDV). Several B-cell epitope (B2T) dendrimers, harboring a major FMDV antigenic B-cell site in VP1 protein, are covalently linked to heterotypic T-cell epitopes from 3A and/or 3D proteins, and elicited consistent levels of neutralizing antibodies and IFN-gamma-producing cells in pigs. To address the contribution of the highly polymorphic nature of the porcine MHC (SLA, swine leukocyte antigen) on the immunogenicity of B2T dendrimers, low-resolution (Lr) haplotyping was performed. We looked for possible correlations between particular Lr haplotypes with neutralizing antibody and T-cell responses induced by B2T peptides. In this study, 63 pigs immunized with B2T dendrimers and 10 non-immunized (control) animals are analyzed. The results reveal a robust significant correlation between SLA class-II Lr haplotypes and the T-cell response. Similar correlations of T-cell response with SLA class-I Lr haplotypes, and between B-cell antibody response and SLA class-I and SLA class-II Lr haplotypes, were only found when the sample was reduced to animals with Lr haplotypes represented more than once. These results support the contribution of SLA class-II restricted T-cells to the magnitude of the T-cell response and to the antibody response evoked by the B2T dendrimers, being of potential value for peptide vaccine design against FMDV.
PB MDPI
SN 2076-393X
YR 2020
FD 2020-09-08
LK https://hdl.handle.net/10016/34107
UL https://hdl.handle.net/10016/34107
LA eng
NO This work was supported by the Spanish Ministry of Science, Innovation and Universities (grant AGL2017-89097-C2 to F.S. and D.A.; AGL2016-76445-R to E.B.; PGC2018-096849-B-I00 to Y.S. and D.C.), Comunidad de Madrid co-financed with ECFEDER funds (P2018/BAA-4370 to F.S. and E.B.) and  Generalitat de Catalunya (2009SGR492 to D.A.). Work at Centro de Biología Molecular “Severo Ochoa” and at UPF was supported by Fundación Ramón Areces and by the Maria de Maeztu Program of the Spanish Ministry of Science, Innovation and Universities, respectively. R. Cañas-Arranz and M. Forner were holders of a PhD fellowship from the Spanish Ministry of Science, Innovation and University (FPI programme).
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RD 27 jul. 2024