RT Journal Article
T1 Preparation and characterization of plasma-derived fibrin hydrogels modified by alginate di-aldehyde
A1 Sanz Horta, Raúl
A1 Matesanz Fernandez-Arias, Ana
A1 Jorcano Noval, José Luis
A1 Velasco Bayón, Diego
A1 Acedo Gallardo, Pablo
A1 Gallardo, Alberto
A1 Reinecke, Helmut
AB Fibrin hydrogels are one of the most popular scaffolds used in tissue engineering due to their excellent biological properties. Special attention should be paid to the use of human plasma-derived fibrin hydrogels as a 3D scaffold in the production of autologous skin grafts, skeletal muscle regeneration and bone tissue repair. However, mechanical weakness and rapid degradation, which causes plasma-derived fibrin matrices to shrink significantly, prompted us to improve their stability. In our study, plasma-derived fibrin was chemically bonded to oxidized alginate (alginate di-aldehyde, ADA) at 10%, 20%, 50% and 80% oxidation, by Schiff base formation, to produce natural hydrogels for tissue engineering applications. First, gelling time studies showed that the degree of ADA oxidation inhibits fibrin polymerization, which we associate with fiber increment and decreased fiber density; moreover, the storage modulus increased when increasing the final volume of CaCl2 (1% w/v) from 80 µL to 200 µL per milliliter of hydrogel. The contraction was similar in matrices with and without human primary fibroblasts (hFBs). In addition, proliferation studies with encapsulated hFBs showed an increment in cell viability in hydrogels with ADA at 10% oxidation at days 1 and 3 with 80 µL of CaCl2; by increasing this compound (CaCl2), the proliferation does not significantly increase until day 7. In the presence of 10% alginate oxidation, the proliferation results are similar to the control, in contrast to the sample with 20% oxidation whose proliferation decreases. Finally, the viability studies showed that the hFB morphology was maintained regardless of the degree of oxidation used; however, the quantity of CaCl2 influences the spread of the hFBs
PB MDPI
SN 1422-0067
YR 2022
FD 2022-04-02
LK https://hdl.handle.net/10016/36420
UL https://hdl.handle.net/10016/36420
LA eng
NO This article belongs to the Special Issue Biomaterials and Regenerative Medicine.
NO This research was supported by Programa de Actividades de I + D entre Grupos de Investigación de la Comunidad de Madrid, S2018/BAA-4480, Biopieltec-CM, Programa Estatal de I + D + i Orientada a los Retos de la Sociedad, RTI2018-101627-B-I00, and Cátedra Fundación Ramón Areces.
DS e-Archivo
RD 1 jul. 2024