RT Journal Article
T1 Vertically scanned laser sheet microscopy
A1 Dong, Di
A1 Arranz, Alicia
A1 Zhu, Shouping
A1 Yang, Yujie
A1 Wang, Jun
A1 Shen, Chen
A1 Tian, Jie
A1 Ripoll Lorenzo, Jorge
AB Laser sheet microscopy is a widely used imaging technique for imaging the three-dimensional distribution of a fluorescence signal in fixed tissue or small organisms. In laser sheet microscopy, the stripe artifacts caused by high absorption or high scattering structures are very common, greatly affecting image quality. To solve this problem, we report here a two-step procedure which consists of continuously acquiring laser sheet images while vertically displacing the sample, and then using the variational stationary noise remover (VSNR) method to further reduce the remaining stripes. Images from a cleared murine colon acquired with a vertical scan are compared with common stitching procedures demonstrating that vertically scanned light sheet microscopy greatly improves the performance of current light sheet microscopy approaches without the need for complex changes to the imaging setup and allows imaging of elongated samples, extending the field of view in the vertical direction.
PB Society of Photo-Optical Instrumentation Engineers (SPIE)
SN 1083-3668
SN 1560-2281 (online)
YR 2014
FD 2014-10-01
LK https://hdl.handle.net/10016/19598
UL https://hdl.handle.net/10016/19598
LA eng
NO This work was supported in part by the Bill and Melinda GatesFoundation, the National Basic Research Program of China (973Program) under Grant No. 2011CB707700, the National NaturalScience Foundation of China under Grant No. 81227901,81027002, 61231004, and 81101095, the Fellowship forYoung International Scientists of the Chinese Academy ofSciences under Grant No. 2010Y2GA03, and the InstrumentDeveloping Project of the Chinese Academy of Sciencesunder Grant No. YZ201164. A. Arranz acknowledges supportfrom the Marie Curie Intra-European Fellowship programIEF-2010-275137. J.R. acknowledges support from EC FP7IMI project PREDICT-TB, the EC FP7 CIG grant HIGHTHROUGHPUTTOMO, and the Spanish MINECO projectgrant FIS2013-41802-R MESO-IMAGING.
DS e-Archivo
RD 31 may. 2024