Effect of illumination level [18F]FDG-PET brain uptake in free moving mice

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dc.contributor.author Francisco, Alexandra de
dc.contributor.author Sierra Palomares, Yolanda
dc.contributor.author Felioe, María
dc.contributor.author Calle, Daniel
dc.contributor.author Desco Menéndez, Manuel
dc.contributor.author Cusso Mula, Lorena
dc.date.accessioned 2022-05-11T08:49:01Z
dc.date.available 2022-05-11T08:49:01Z
dc.date.issued 2021-05-13
dc.identifier.bibliographicCitation de Francisco, A., Sierra-Palomares, Y., Felipe, M., Calle, D., Desco, M., & Cussó, L. (2021). Effect of illumination level [18F]FDG-PET brain uptake in free moving mice. PLOS ONE, 16(5), e0251454.
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10016/34761
dc.description.abstract In both clinical and preclinical scenarios, 2-deoxy-2[18F]fluoro-D-glucose ([18F]FDG) is the radiotracer most widely used to study brain glucose metabolism with positron emission tomography (PET). In clinical practice, there is a worldwide standardized protocol for preparing patients for [18F]FDG-PET studies, which specifies the room lighting. However, this standard is typically not observed in the preclinical field, although it is well known that animal handling affects the biodistribution of [18F]FDG. The present study aimed to evaluate the effect of ambient lighting on brain [18F]FDG uptake in mice. Two [18F]FDG-PET studies were performed on each animal, one in light and one in dark conditions. Thermal video recordings were acquired to analyse animal motor activity in both conditions. [18F]FDGPET images were analysed with the Statistical Parametric Mapping method. The results showed that [18F]FDG uptake is higher in darkness than in light condition in mouse nucleus accumbens, hippocampus, midbrain, hindbrain, and cerebellum. The SPM analysis also showed an interaction between the illumination condition and the sex of the animal. Mouse activity was significantly different (p = 0.01) between light conditions (632 ± 215 s of movement) and dark conditions (989 ± 200 s), without significant effect of sex (p = 0.416). We concluded that room illumination conditions during [18F]FDG uptake in mice affected the brain [18F]FDG biodistribution. Therefore, we highlight the importance to control this factor to ensure more reliable and reproducible mouse brain [18F]FDG-PET results.
dc.description.sponsorship This work was partially supported by the Comunidad de Madrid (S2017/BMD-3867 RENIMCM), and it was co-financed by the European Structural and Investment Fund. And by Ministerio de Ciencia e Innovación, Instituto de Salud Carlos III (PT20/00044), cofunded by European Union, European Regional Development Fund (ERDF),"A way of making Europe". The CNIC is supported by the ISCIII, the Ministerio de Ciencia e Innovación.
dc.format.extent 8
dc.language.iso eng
dc.publisher Public Library of Science
dc.rights © 2021 de Francisco et al.
dc.rights Atribución 3.0 España
dc.rights.uri http://creativecommons.org/licenses/by/3.0/es/
dc.title Effect of illumination level [18F]FDG-PET brain uptake in free moving mice
dc.type article
dc.subject.eciencia Medicina
dc.identifier.doi https://doi.org/10.1371/journal.pone.0251454
dc.rights.accessRights openAccess
dc.type.version publishedVersion
dc.identifier.publicationfirstpage 1
dc.identifier.publicationissue 5
dc.identifier.publicationlastpage 8
dc.identifier.publicationtitle PLoS One
dc.identifier.publicationvolume 16
dc.identifier.uxxi AR/0000028655
dc.contributor.funder Comunidad de Madrid
dc.contributor.funder Ministerio de Ciencia e Innovación (España)
dc.affiliation.dpto UC3M. Departamento de Bioingeniería
dc.affiliation.grupoinv UC3M. Grupo de Investigación: Biomedical Imaging and Instrumentation Group
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