Elastin-plasma hybrid hydrogels for skin tissue engineering

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dc.contributor.author Stojic, Marija
dc.contributor.author Ródenas-Rochina, Joaquín
dc.contributor.author López-Donaire, María Luisa
dc.contributor.author González De Torre, Israel
dc.contributor.author González Pérez, Miguel
dc.contributor.author Rodríguez-Cabello, José Carlos
dc.contributor.author Vojtová, Lucy
dc.contributor.author Jorcano Noval, José Luis
dc.contributor.author Velasco Bayón, Diego
dc.date.accessioned 2021-09-30T09:48:06Z
dc.date.available 2021-09-30T09:48:06Z
dc.date.issued 2021-07-01
dc.identifier.bibliographicCitation Stojic, M., Ródenas-Rochina, J., López-Donaire, M. L., González De Torre, I., González Pérez, M., Rodríguez-Cabello, J. C., Vojtová, L., Jorcano, J. L. & Velasco, D. (2021). Elastin-Plasma Hybrid Hydrogels for Skin Tissue Engineering. Polymers, 13(13), 2114.
dc.identifier.issn 2073-4360
dc.identifier.uri http://hdl.handle.net/10016/33345
dc.description This article belongs to the Special Issue Functional Polymer Gels for Advanced Applications in Biomedicine, Energy and Optoelectronics.
dc.description.abstract Dermo-epidermal equivalents based on plasma-derived fibrin hydrogels have been extensively studied for skin engineering. However, they showed rapid degradation and contraction over time and low mechanical properties which limit their reproducibility and lifespan. In order to achieve better mechanical properties, elasticity and biological properties, we incorporated a elastin-like recombinamer (ELR) network, based on two types of ELR, one modified with azide (SKS-N3) and other with cyclooctyne (SKS-Cyclo) chemical groups at molar ratio 1:1 at three different SKS (serine-lysine-serine sequence) concentrations (1, 3, and 5 wt.%), into plasma-derived fibrin hydrogels. Our results showed a decrease in gelation time and contraction, both in the absence and presence of the encapsulated human primary fibroblasts (hFBs), higher mechanical properties and increase in elasticity when SKSs content is equal or higher than 3%. However, hFBs proliferation showed an improvement when the lowest SKS content (1 wt.%) was used but started decreasing when increasing SKS concentration at day 14 with respect to the plasma control. Proliferation of human primary keratinocytes (hKCs) seeded on top of the hybrid-plasma hydrogels containing 1 and 3% of SKS showed no differences to plasma control and an increase in hKCs proliferation was observed for hybrid-plasma hydrogels containing 5 wt.% of SKS. These promising results showed the need to achieve a balance between the reduced contraction, the better mechanical properties and biological properties and indicate the potential of using this type of hydrogel as a testing platform for pharmaceutical products and cosmetics, and future work will elucidate their potential.
dc.description.sponsorship The authors are grateful for the funding from the Spanish Government (FPU15-00448, PID2019-110709RB-100, RED2018-102417-T), Junta de Castilla y León (VA317P18. Infrared2018-UVA06), Interreg. V España Portugal POCTEP (0624_2IQBIONEURO_6_E) and Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León. This research was also funded by Programa de Actividades de I + D entre Grupos de Investigación de la Comunidad de Madrid, S2018/BAA-4480, Biopieltec-CM; by Programa Estatal de I + D + i Orientada a los Retos de la So-ciedad, RTI2018-101627-B-I00; by Programa de Apoyo a la Realización de Proyectos Interdisciplinares de I + D para Jóvenes Investigadores de la Universidad Carlos III de Madrid (project: BIOMASKIN); and by Cátedra Fundación Ramón Areces.
dc.format.extent 23
dc.language.iso eng
dc.publisher MDPI
dc.rights © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
dc.rights Atribución 3.0 España
dc.rights.uri http://creativecommons.org/licenses/by/3.0/es/
dc.subject.other Bilayered in vitro skin substitutes
dc.subject.other Elastin like recombinamers
dc.subject.other Human plasma-derived fibrin hydrogels
dc.subject.other Fibrin hydrogels
dc.subject.other Hybrid plasma-elastin hydrogels
dc.subject.other Bioengineered skin
dc.subject.other Skin tissue engineering
dc.title Elastin-plasma hybrid hydrogels for skin tissue engineering
dc.type article
dc.subject.eciencia Aeronáutica
dc.subject.eciencia Biología y Biomedicina
dc.identifier.doi https://doi.org/10.3390/polym13132114
dc.rights.accessRights openAccess
dc.relation.projectID Comunidad de Madrid. S2018/BAA-4480
dc.relation.projectID Gobierno de España. RTI2018-101627-B-I00
dc.relation.projectID Gobierno de España. FPU15-00448
dc.type.version publishedVersion
dc.identifier.publicationfirstpage 2114
dc.identifier.publicationissue 13
dc.identifier.publicationtitle Polymers
dc.identifier.publicationvolume 13
dc.identifier.uxxi AR/0000028399
dc.contributor.funder Comunidad de Madrid
dc.contributor.funder Ministerio de Ciencia e Innovación (España)
dc.contributor.funder Ministerio de Educación, Cultura y Deporte (España)
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