Effect of Fibrin Concentration on the In Vitro Production of Dermo-Epidermal Equivalents

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dc.contributor.author Montero Simón, Andrés
dc.contributor.author Quilez Lopez, Cristina
dc.contributor.author Valencia Blanco, Leticia
dc.contributor.author Girón, Paula
dc.contributor.author Jorcano Noval, José Luis
dc.contributor.author Velasco Bayón, Diego
dc.date.accessioned 2021-09-30T08:50:39Z
dc.date.available 2021-09-30T08:50:39Z
dc.date.issued 2021-07-01
dc.identifier.bibliographicCitation Montero, A., Quílez, C., Valencia, L., Girón, P., Jorcano, J. L. & Velasco, D. (2021). Effect of Fibrin Concentration on the In Vitro Production of Dermo-Epidermal Equivalents. International Journal of Molecular Sciences, 22(13), 6746.
dc.identifier.issn 1422-0067
dc.identifier.uri http://hdl.handle.net/10016/33344
dc.description This article belongs to the Special Issue Advanced Biomaterials for Wound Healing 2021.
dc.description.abstract Human plasma-derived bilayered skin substitutes were successfully used by our group to produce human-based in vitro skin models for toxicity, cosmetic, and pharmaceutical testing. However, mechanical weakness, which causes the plasma-derived fibrin matrices to contract significantly, led us to attempt to improve their stability. In this work, we studied whether an increase in fibrin concentration from 1.2 to 2.4 mg/mL (which is the useful fibrinogen concentration range that can be obtained from plasma) improves the matrix and, hence, the performance of the in vitro skin cultures. The results show that this increase in fibrin concentration indeed affected the mechanical properties by doubling the elastic moduli and the maximum load. A structural analysis indicated a decreased porosity for the 2.4 mg/mL hydrogels, which can help explain this mechanical behavior. The contraction was clearly reduced for the 2.4 mg/mL matrices, which also allowed for the growth and proliferation of primary fibroblasts and keratinocytes, although at a somewhat reduced rate compared to the 1.2 mg/mL gels. Finally, both concentrations of fibrin gave rise to organotypic skin cultures with a fully differentiated epidermis, although their lifespans were longer (25–35%) in cultures with more concentrated matrices, which improves their usefulness. These systems will allow the generation of much better in vitro skin models for the testing of drugs, cosmetics and chemicals, or even to “personalized” skin for the diagnosis or determination of the most effective treatment possible.
dc.description.sponsorship This research was funded by Programa de Actividades de I+D entre Grupos de Investigación de la Comunidad de Madrid, S2018/BAA-4480, Biopieltec-CM; by Programa Estatal de I+D+i Orientada a los Retos de la Sociedad, RTI2018-101627-B-I00; by Programa de Apoyo a la Realización de Proyectos Interdisciplinares de I+D para Jóvenes Investigadores de la Universidad Carlos III de Madrid (project: BIOMASKIN); and by Cátedra Fundación Ramón Areces.
dc.format.extent 18
dc.language.iso eng
dc.publisher MDPI
dc.rights © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
dc.rights Atribución 3.0 España
dc.rights.uri http://creativecommons.org/licenses/by/3.0/es/
dc.subject.other Fibrin hydrogels
dc.subject.other Plasma-derived fibrin hydrogel
dc.subject.other Bilayered in vitro skin substitutes
dc.subject.other Organotypic skin cultures
dc.subject.other Platelet-poor plasma (PPP)
dc.subject.other Skin tissue engineering
dc.title Effect of Fibrin Concentration on the In Vitro Production of Dermo-Epidermal Equivalents
dc.type article
dc.subject.eciencia Aeronáutica
dc.subject.eciencia Biología y Biomedicina
dc.identifier.doi https://doi.org/10.3390/ijms22136746
dc.rights.accessRights openAccess
dc.relation.projectID Comunidad de Madrid. S2018/BAA-4480
dc.relation.projectID Gobierno de España. RTI2018-101627-B-I00
dc.type.version publishedVersion
dc.identifier.publicationfirstpage 6746
dc.identifier.publicationissue 13
dc.identifier.publicationtitle International Journal of Molecular Sciences
dc.identifier.publicationvolume 22
dc.identifier.uxxi AR/0000028398
dc.contributor.funder Comunidad de Madrid
dc.contributor.funder Ministerio de Ciencia e Innovación (España)
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