A new microfluidic method enabling the generation of multi-layered tissues-on-chips using skin cells as a proof of concept

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dc.contributor.author Valencia Blanco, Leticia
dc.contributor.author Canalejas-Tejero, Víctor
dc.contributor.author Clemente, Miguel
dc.contributor.author Fernaud, Isabel
dc.contributor.author Holgado, Miguel
dc.contributor.author Jorcano Noval, José Luis
dc.contributor.author Velasco Bayón, Diego
dc.date.accessioned 2021-09-29T11:52:39Z
dc.date.available 2021-09-29T11:52:39Z
dc.date.issued 2021-06-23
dc.identifier.bibliographicCitation Valencia, L., Canalejas-Tejero, V., Clemente, M., Fernaud, I., Holgado, M., Jorcano, J. L. & Velasco, D. (2021). A new microfluidic method enabling the generation of multi-layered tissues-on-chips using skin cells as a proof of concept. Scientific Reports, 11, 13160.
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10016/33340
dc.description.abstract Microfluidic-based tissues-on-chips (TOCs) have thus far been restricted to modelling simple epithelia as a single cell layer, but likely due to technical difficulties, no TOCs have been reported to include both an epithelial and a stromal component despite the biological importance of the stroma for the structure and function of human tissues. We present, for the first time, a novel approach to generate 3D multilayer tissue models in microfluidic platforms. As a proof of concept, we modelled skin, including a dermal and an epidermal compartment. To accomplish this, we developed a parallel flow method enabling the deposition of bilayer tissue in the upper chamber, which was subsequently maintained under dynamic nutrient flow conditions through the lower chamber, mimicking the function of a blood vessel. We also designed and built an inexpensive, easy-to-implement, versatile, and robust vinyl-based device that overcomes some of the drawbacks present in PDMS-based chips. Preliminary tests indicate that this biochip will allow the development and maintenance of multilayer tissues, which opens the possibility of better modelling of the complex cell–cell and cell–matrix interactions that exist in and between the epithelium and mesenchyme, allowing for better-grounded tissue modelling and drug screening.
dc.description.sponsorship This work was supported by the "Programa de Actividades de I+D entre Grupos de Investigación de la Comunidad de Madrid" project S2018/BAA-4480, Biopieltec-CM and the Cátedra Fundación Ramón Areces.
dc.format.extent 14
dc.language.iso eng
dc.publisher Nature Research
dc.rights © The Author(s) 2021.
dc.rights Atribución 3.0 España
dc.rights.uri http://creativecommons.org/licenses/by/3.0/es/
dc.subject.other Biomedical Engineering
dc.subject.other Fluidics
dc.subject.other Lab-on-a-chip
dc.title A new microfluidic method enabling the generation of multi-layered tissues-on-chips using skin cells as a proof of concept
dc.type article
dc.subject.eciencia Biología y Biomedicina
dc.identifier.doi https://doi.org/10.1038/s41598-021-91875-z
dc.rights.accessRights openAccess
dc.relation.projectID Comunidad de Madrid. S2018/BAA-4480
dc.type.version publishedVersion
dc.identifier.publicationfirstpage 1
dc.identifier.publicationissue 13160
dc.identifier.publicationlastpage 14
dc.identifier.publicationtitle Scientific Reports
dc.identifier.publicationvolume 11
dc.identifier.uxxi AR/0000028259
dc.contributor.funder Comunidad de Madrid
dc.affiliation.dpto UC3M. Departamento de Bioingeniería
dc.affiliation.grupoinv UC3M. Grupo de Investigación: Tissue Engineering and Regenerative Medicine (TERMeG)
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