Two mechanisms regulate directional cell growth in Arabidopsis lateral roots

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Show simple item record Kirchhelle, Charlotte García González, Daniel Irani, Niloufer G. Jerusalem, Antoine Moore, Ian 2020-11-16T16:17:12Z 2020-11-16T16:17:12Z 2019-07-29
dc.identifier.bibliographicCitation Kirchhelle, C., Garcia-Gonzalez, D., Irani, N. G., Jérusalem, A., & Moore, I. (2019). Two mechanisms regulate directional cell growth in Arabidopsis lateral roots. ELife, 8
dc.identifier.issn 2050-084X
dc.description.abstract Morphogenesis in plants depends critically on directional (anisotropic) growth. This occurs principally perpendicular to the net orientation of cellulose microfibrils (CMFs), which is in turn controlled by cortical microtubules (CMTs). In young lateral roots of Arabidopsis thaliana, growth anisotropy also depends on RAB-A5c, a plant-specific small GTPase that specifies a membrane trafficking pathway to the geometric edges of cells. Here we investigate the functional relationship between structural anisotropy at faces and RAB-A5c activity at edges during lateral root development. We show that surprisingly, inhibition of RAB-A5c function is associated with increased CMT/CMF anisotropy. We present genetic, pharmacological, and modelling evidence that this increase in CMT/CMF anisotropy partially compensates for loss of an independent RAB-A5c-mediated mechanism that maintains anisotropic growth in meristematic cells. We show that RAB-A5c associates with CMTs at cell edges, indicating that CMTs act as an integration point for both mechanisms controlling cellular growth anisotropy in lateral roots
dc.description.sponsorship This work was supported by Leverhulme Trust grant RPG-2014–276 and a John Fell Award to IM, BBSRC grant BB/P01979X/1 to IM, CK, and AJ, and a BBSRC doctoral training award, Clarendon Scholarship, and Leverhulme Early Career Fellowship ECF-2017–483 to CK. DG-G and AJ acknowledge funding from the European Union’s Seventh Framework Programme (FP7 2007–2013) ERC Grant Agreement No. 306587.
dc.language.iso eng
dc.publisher eLife Sciences Publications Ltd
dc.rights Reconocimiento 3.0 España
dc.subject.other Katanin
dc.subject.other Microtubule
dc.subject.other Cytokinesis
dc.title Two mechanisms regulate directional cell growth in Arabidopsis lateral roots
dc.type article PeerReviewed
dc.subject.eciencia Ingeniería Mecánica
dc.subject.eciencia Biología y Biomedicina
dc.rights.accessRights openAccess
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/306587
dc.type.version publishedVersion
dc.identifier.publicationfirstpage 1
dc.identifier.publicationissue 8, e47988
dc.identifier.publicationlastpage 26
dc.identifier.publicationtitle eLife
dc.identifier.uxxi AR/0000024984
dc.contributor.funder European Commission
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