X-ray-based virtual slicing of TB-infected lungs

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dc.contributor.author Ortega Gil, Ana
dc.contributor.author Vaquero López, Juan José
dc.contributor.author González Arjona, Mario
dc.contributor.author Rullas, Joaquín
dc.contributor.author Muñoz Barrutia, María Arrate
dc.date.accessioned 2020-01-14T12:00:57Z
dc.date.available 2020-01-14T12:00:57Z
dc.date.issued 2019-12-18
dc.identifier.bibliographicCitation Ortega-Gil, A., Vaquero, J.J., Gonzalez-Arjona, M., Rullas, J., Muñoz-Barrutia, A.(2019). X-ray-based virtual slicing of TB-infected lungs. Scientific Reports, 9, 19404.
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10016/29451
dc.description.abstract Hollow organs such as the lungs pose a considerable challenge for post-mortem imaging in preclinical research owing to their extremely low contrast and high structural complexity. The aim of our study was to enhance the contrast of tuberculosis lesions for their stratification by 3D x-ray&-based virtual slicing. Organ samples were taken from five control and five tuberculosis-infected mice. Micro-Computed Tomography (CT) scans of the subjects were acquired in vivo (without contrast agent) and post-mortem (with contrast agent). The proposed contrast-enhancing technique consists of x-ray contrast agent uptake (silver nitrate and iodine) by immersion. To create the histology ground-truth, the CT scan of the paraffin block guided the sectioning towards specific planes of interest. The digitalized histological slides reveal the presence, extent, and appearance of the contrast agents in lung structures and organized aggregates of immune cells. These findings correlate with the contrast-enhanced micro-CT slice. The abnormal densities in the lungs due to tuberculosis disease are concentrated in the right tail of the lung intensity histograms. The increase in the width of the right tail (~376%) indicates a contrast enhancement of the details of the abnormal densities. Postmortem contrast agents enhance the x-ray attenuation in tuberculosis lesions to allow 3D visualization by polychromatic x-ray CT, providing an advantageous tool for virtual slicing of whole lungs. The proposed contrast-enhancing technique combined with computational methods and the diverse micro-CT modalities will open the doors to the stratification of lesion types associated with infectious diseases.
dc.description.sponsorship The research leading to these results received funding from the Innovative Medicines Initiative (www.imi.europa.eu) Joint Undertaking under grant agreement no. 115337, whose resources comprise funding from the European Union Seventh Framework Programme (FP7/2007–2013) and EFPIA companies in kind contribution. This work was partially funded by projects RTC-2015-3772-1, TEC2015-73064-EXP and TEC2016-78052-R from the Spanish Ministry of Economy, TOPUS S2013/MIT-3024 project from the regional government of Madrid and by the Department of Health, UK. This study (was supported by the Instituto de Salud Carlos III (Plan Estatal de I + D + i 2013–2016) and co-financed by the European Social Fund (ESF) “ESF investing in your future”. The authors thank Dr.Guembe from CIMA-Universidad de Navarra for preparing and staining the tissue sections, and to Dr. Guerrero-Aspizua and Prof. Conti of the Department of Bioengineering, Universidad Carlos III de Madrid for the pathology evaluation.
dc.format.extent 12
dc.language.iso eng
dc.publisher Nature Publishing Group
dc.rights © 2019 The Author(s).
dc.rights Atribución-NoComercial-SinDerivadas 3.0 España
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.title X-ray-based virtual slicing of TB-infected lungs
dc.type article
dc.subject.eciencia Biología y Biomedicina
dc.identifier.doi https://doi.org/10.1038/s41598-019-55986-y
dc.rights.accessRights openAccess
dc.relation.projectID Gobierno de España. RTC-2015-3772-1
dc.relation.projectID Gobierno de España. TEC2015-73064-EXP
dc.relation.projectID Gobierno de España. TEC2016-78052-R
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/115337
dc.type.version publishedVersion
dc.identifier.publicationissue 9
dc.identifier.publicationtitle Scientific Reports
dc.identifier.uxxi AR/0000024187
dc.contributor.funder European Commission
dc.contributor.funder Ministerio de Economía y Competitividad (España)
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