Cellular behaviour of bone marrow stromal cells on modified Ti-Nb surfaces

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dc.contributor.author Ureña Alcázar, Julia María
dc.contributor.author Tsipas, Sophia Alexandra
dc.contributor.author Jiménez Morales, Antonia
dc.contributor.author Gordo Odériz, Elena
dc.contributor.author Rainer, Detsch
dc.contributor.author Boccaccini, Aldo
dc.date.accessioned 2018-09-17T08:37:02Z
dc.date.available 2020-02-15T00:00:03Z
dc.date.issued 2018-02-15
dc.identifier.bibliographicCitation Materials & Design, 140, (2018), pp. 452-459.
dc.identifier.issn 0264-1275
dc.identifier.uri http://hdl.handle.net/10016/27417
dc.description.abstract The cellular behaviour of bone marrow stromal cells on titanium surfaces modified by niobium diffusion is presented in order to test their osteogenic differentiation response after culturing for 21 days. The surface modification of Ti substrates produced by powder metallurgy was performed through niobium diffusion treatments. Ti-Nb exhibited a beta-Ti surface together with a microstructural (beta / alpha + beta / alpha) and compositional (Ti-Nb) gradient which enhances hardness, wear resistance and lowers the elastic modulus making it more similar to the human bone. Furthermore, the Ti-Nb-NH4Cl by means of the activating agent achieved three times the hardness of Ti together with a porous surface. The in vitro osteogenic differentiation response of bonemarrowstromal cells on both Ti-Nb surfaces indicated the positive cell-material interaction. The osteogenic differentiation of cells was successful after 21 days, considering the positive response in terms of increased cell viability, lactate dehydrogenase-(LDH) activity, alkaline phosphatase-(ALP) activity expression (osteogenicmarker) and bone-like nodules deposition by ST-2 cells as a bone mineralization cue. Therefore, the positive effect of a low elastic modulus Ti-Nb surface and a porous nitride TiNbNH4Cl with suitable wettability and average roughness values on the osteogenic differentiation response of bone marrow stromal cells is demonstrated. (C) 2017 Elsevier Ltd. All rights reserved.
dc.description.sponsorship The authors would like to thank the funding provided for this research by the Regional Government of Madrid (program MULTIMAT-CHALLENGE-CM, ref. S2013/MIT-2862).
dc.format.extent 7
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof http://hdl.handle.net/10016/27609
dc.rights © 2017 Elsevier Ltd. All rights reserved.
dc.rights Atribución-NoComercial-SinDerivadas 3.0 España
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.other Beta-Gradient titanium
dc.subject.other Surface modification
dc.subject.other Powder technology
dc.subject.other Osteogenic differentiation
dc.subject.other Bone mineralization
dc.title Cellular behaviour of bone marrow stromal cells on modified Ti-Nb surfaces
dc.type article
dc.subject.eciencia Materiales
dc.identifier.doi https://doi.org/10.1016/j.matdes.2017.12.006
dc.rights.accessRights openAccess
dc.relation.projectID Comunidad de Madrid. S2013/MIT-2862/MULTIMAT-CHALLENGE-CM
dc.type.version acceptedVersion
dc.identifier.publicationfirstpage 452
dc.identifier.publicationlastpage 459
dc.identifier.publicationtitle Materials & Desing
dc.identifier.publicationvolume 140
dc.identifier.uxxi AR/0000021061
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