Polarization-sensitive optical projection tomography for muscle fiber imaging

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dc.contributor.author Fang, Mengjie
dc.contributor.author Dong, Di
dc.contributor.author Zeng, Chaoting
dc.contributor.author Liang, Xiao
dc.contributor.author Yang, Xin
dc.contributor.author Arranz de Miguel, Alicia
dc.contributor.author Ripoll Lorenzo, Jorge
dc.contributor.author Hui, Hui
dc.contributor.author Tian, Jie
dc.date.accessioned 2018-01-22T12:33:30Z
dc.date.available 2018-01-22T12:33:30Z
dc.date.issued 2016-01-11
dc.identifier.bibliographicCitation Scientific reports, 6 (19241), pp. 1-8
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10016/26148
dc.description.abstract Optical projection tomography (OPT) is a tool used for three-dimensional imaging of millimeter-scale biological samples, with the advantage of exhibiting isotropic resolution typically in the micron range. OPT can be divided into two types: transmission OPT (tOPT) and emission OPT (eOPT). Compared with eOPT, tOPT discriminates different tissues based on their absorption coefficient, either intrinsic or after specific staining. However, it fails to distinguish muscle fibers whose absorption coefficients are similar to surrounding tissues. To circumvent this problem, in this article we demonstrate a polarization sensitive OPT system which improves the detection and 3D imaging of muscle fibers by using polarized light. We also developed image acquisition and processing protocols that, together with the system, enable the clear visualization of muscles. Experimental results show that the muscle fibers of diaphragm and stomach, difficult to be distinguished in regular tOPT, were clearly displayed in our system, proving its potential use. Moreover, polarization sensitive OPT was fused with tOPT to investigate the stomach tissue comprehensively. Future applications of polarization sensitive OPT could be imaging other fiberlike structures such as myocardium or other tissues presenting high optical anisotropy.
dc.description.sponsorship This work is supported by the National Basic Research Program of China (973 Program) under Grant 2011CB707700, the National Natural Science Foundation of China under Grant No. 81227901, 61231004, 81501616, 81301346, 81527805 the Chinese Academy of Sciences Fellowship for Young Foreign Scientists under Grant No. 2010Y2GA03, 2013Y1GA0004, the Chinese Academy of Sciences Visiting Professorship for Senior International Scientists under Grant No. 2012T1G0036, 2013T1G0013, the Instrument Developing Project of the Chinese Academy of Sciences under Grant No. YZ201502, YZ201457 and the Strategic Priority Research Program (B) of Chinese Academy of Sciences (XDB02060010). A. Arranz acknowledges support from the Marie Curie Intra-European Fellowship program IEF-2010-275137. J.R. acknowledges support from EC FP7 IMI project PREDICT-TB, the EC FP7 CIG grant HIGH-THROUGHPUT TOMO, and the Spanish MINECO project grant FIS2013-41802-R MESO-IMAGING.
dc.format.extent 8
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Nature Publishing Group
dc.rights © 2015 Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License.
dc.rights Atribución 3.0 España
dc.rights.uri http://creativecommons.org/licenses/by/3.0/es/
dc.subject.other 3-D reconstruction
dc.subject.other Optical imaging
dc.title Polarization-sensitive optical projection tomography for muscle fiber imaging
dc.type article
dc.subject.eciencia Biología y Biomedicina
dc.identifier.doi https://doi.org/10.1038/srep19241
dc.rights.accessRights openAccess
dc.relation.projectID info:eu-repo/grantAgreement/EC/ IEF/2010-275137
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/115337
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/333632
dc.relation.projectID Gobierno de España. FIS2013 41802 R (MESO IMAGING)
dc.type.version publishedVersion
dc.identifier.publicationfirstpage 1
dc.identifier.publicationlastpage 8
dc.identifier.publicationtitle Scientific reports
dc.identifier.publicationvolume 6 (19241)
dc.identifier.uxxi AR/0000020742
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