Sponsor:
The work on this review article has received funding from the Deutsche Forschungsgemeinschaft (DFG), Germany (Leibniz Prize 2013; NT 3/10 1) and the Federal Ministry of Education and Research (BMBF), Photonic Science Germany, Tech2See 13N12623/ 4. J.R. acknowledges support from the European Commission FP7 CIG grant HIGH THROUGH PUT TOMO, and Spanish MINECO grant MESO IMAGING FIS2013 41802 R.
Project:
Gobierno de España. FIS2013 41802 R (MESO IMAGING) info:eu-repo/grantAgreement/EC/FP7/333632
The past decade marked an optical revolution in biology: an unprecedented number of optical techniques were developed and adopted for biological exploration, demonstrating increasing interest in optical imaging and in vivo interrogations. Optical methods have The past decade marked an optical revolution in biology: an unprecedented number of optical techniques were developed and adopted for biological exploration, demonstrating increasing interest in optical imaging and in vivo interrogations. Optical methods have become faster and have reached nanoscale resolution, and are now complemented by optoacoustic (photoacoustic) methods capable of imaging whole specimens in vivo. Never before were so many optical imaging barriers broken in such a short time-frame: with new approaches to optical microscopy and mesoscopy came an increased ability to image biology at unprecedented speed, resolution, and depth. This review covers the most relevant techniques for imaging in developmental biology, and offers an outlook on the next steps for these technologies and their applications.[+][-]