Photopatterned Antibodies for Selective Cell Attachment

e-Archivo Repository

Show simple item record de Almeida Custódio, Catarina San Miguel Arnanz, Verónica Gropeanu, Radu A. Gropeanu, Mihaela Wirkner, Melanie Reis, Rui L. Mano, João F. del Campo, Aránzazu 2017-05-10T08:53:32Z 2017-05-10T08:53:32Z 2014-08-26
dc.identifier.bibliographicCitation Langmuir, 2014, 30 (33), pp. 10066-10071.
dc.identifier.issn 0743-7463
dc.description.abstract We present a phototriggerable system that allows for the spatiotemporal controlled attachment of selected cell types to a biomaterial using immobilized antibodies that specifically target individual cell phenotypes. o-Nitrobenzyl caged biotin was used to functionalize chitosan membranes and mediate site-specific coupling of streptavidin and biotinylated antibodies after light activation. The ability of this system to capture and immobilize specific cells on a surface was tested using endothelial-specific biotinylated antibodies and nonspecific ones as controls. Homogeneous patterned monolayers of human umbilical vein endothelial cells were obtained on CD31-functionalized surfaces. This is a simple and generic approach that is applicable to other ligands, materials, and cell types and shows the flexibility of caged ligands to trigger and control the interaction between cells and biomaterials.
dc.description.sponsorship We thank Martina Knecht (MPIP) for help with the synthesis of caged biotin and Dr. Ron Unger and Prof. C. J. Kirkpatrick (University Clinic Mainz, RepairLab) for providing HUVECs. C.A.C. acknowledges funding support from the Portuguese Foundation for Science and Technology (FCT) (fellowship SFRH/BD/61390/2009) and from the International Max-Planck Research School in Mainz. The research leading to these results has received funding from the European Union’s Seventh Framework Programme (FP7/2007-2013) under grant agreement no. REGPOT-CT2012-316331-POLARIS.
dc.description.sponsorship European Community's Seventh Framework Program
dc.format.extent 6
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher American Chemical Society
dc.rights © 2014 American Chemical Society
dc.title Photopatterned Antibodies for Selective Cell Attachment
dc.type article
dc.subject.eciencia Materiales
dc.subject.eciencia Química
dc.rights.accessRights openAccess
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/316331
dc.type.version acceptedVersion
dc.identifier.publicationfirstpage 10066
dc.identifier.publicationissue 33
dc.identifier.publicationlastpage 10071
dc.identifier.publicationtitle Langmuir
dc.identifier.publicationvolume 30
dc.identifier.uxxi AR/0000019823
 Find Full text

Files in this item

*Click on file's image for preview. (Embargoed files's preview is not supported)

This item appears in the following Collection(s)

Show simple item record