Photoactivatable Caged Cyclic RGD Peptide for Triggering Integrin Binding and Cell Adhesion to Surfaces

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dc.contributor.author Wirkner, Melanie
dc.contributor.author Weis, Simone
dc.contributor.author San Miguel Arnanz, Verónica
dc.contributor.author Álvarez, Marta
dc.contributor.author Gropeanu, Radu A.
dc.contributor.author Salierno, Marcelo
dc.contributor.author Sartoris, Anne
dc.contributor.author Unger, Ronald D.
dc.contributor.author Kirkpatrick, Charles James
dc.contributor.author del Campo, Aránzazu
dc.date.accessioned 2017-05-08T10:17:54Z
dc.date.available 2017-05-08T10:17:54Z
dc.date.issued 2011-11-18
dc.identifier.bibliographicCitation ChemBioChem, 2011, 12 (17), pp. 2623-2629.
dc.identifier.issn 1439-4227
dc.identifier.uri http://hdl.handle.net/10016/24535
dc.description.abstract We report the synthesis and properties of a photoactivatable caged RGD peptide and its application for phototriggering integrin- and cell-binding to surfaces. We analysed in detail 1) the differences in the integrin-binding affinity of the caged and uncaged forms by quartz crystal microbalance (QCM) studies, 2) the efficiency and yield of the photolytic uncaging reaction, 3) the biocompatibility of the photolysis by-products and irradiation conditions, 4) the possibility of site, temporal and density control of integrin-binding and therefore human cell attachment, and 5) the possibility of in situ generation of cell patterns and cell gradients by controlling the UV exposure. These studies provide a clear picture of the potential and limitations of caged RGD for integrin-mediated cell adhesion and demonstrate the application of this approach to the control and study of cell interactions and responses.
dc.description.sponsorship The authors thank Martina Knecht for help in the synthetic part and Alexander Specht and Maurice Goeldner (University Strasbourg) for previous work in the caged RGD and discussion of the photolysis experiments. AdC and VSM thank the DFG for financial support (DFG-ANR project, CA880-3). AdC and MW thank the Materials World Network (DFG AOBJ 569628) for financial support.
dc.format.extent 7
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher WILEY-VCH
dc.rights © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
dc.title Photoactivatable Caged Cyclic RGD Peptide for Triggering Integrin Binding and Cell Adhesion to Surfaces
dc.type article
dc.subject.eciencia Materiales
dc.subject.eciencia Química
dc.identifier.doi https://dx.doi.org/10.1002/cbic.201100437
dc.rights.accessRights openAccess
dc.type.version submittedVersion
dc.identifier.publicationfirstpage 2623
dc.identifier.publicationissue 17
dc.identifier.publicationlastpage 2629
dc.identifier.publicationtitle ChemBioChem
dc.identifier.publicationvolume 12
dc.identifier.uxxi AR/0000019808
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