3D imaging in CUBIC-cleared mouse heart tissue: going deeper

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dc.contributor.author Nehrhoff, Imke
dc.contributor.author Bocancea, Diana
dc.contributor.author Vaquero, Javier
dc.contributor.author Vaquero López, Juan José
dc.contributor.author Ripoll Lorenzo, Jorge
dc.contributor.author Desco Menéndez, Manuel
dc.contributor.author Gómez Gaviro, María Victoria
dc.date.accessioned 2020-05-05T13:22:25Z
dc.date.available 2020-05-05T13:22:25Z
dc.date.issued 2016-08
dc.identifier.bibliographicCitation Biomedical Optics Express, vol. 7, n. 9, Sept. 2016, 3716-3720
dc.identifier.issn 2156-7085
dc.identifier.uri http://hdl.handle.net/10016/23519
dc.description.abstract The ability to acquire high resolution 3D images of the heart enables to study heart diseases more in detail. In this work, the CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) clearing protocol was optimized for thick mouse heart sections to enhance the penetration depth of the confocal microscope lasers into the tissue. In addition, the optimized CUBIC clearing of the heart enhances antibody penetration into the tissue by a factor of five. The present protocol enables deep 3D high-quality image acquisition in the heart allowing a much more accurate assessment of the cellular and structural changes that underlie heart diseases.
dc.description.sponsorship Human Frontiers Scientific Program (RGP0004/2013); EC FP7 CIG grant HIGH THROUGHPUT TOMO; Comunidad de Madrid BRADE (S2013/ICE-2958); MINECO. Ramón y Cajal program (RYC 2010-05838); MINECO, ISCIII-Fondos FEDER (PI12/01580).
dc.description.sponsorship European Community's Seventh Framework Program
dc.format.mimetype application/pdf
dc.language.iso eng
dc.rights © 2016 Optical Society of America
dc.title 3D imaging in CUBIC-cleared mouse heart tissue: going deeper
dc.type article
dc.subject.eciencia Biología y Biomedicina
dc.identifier.doi https://doi.org/10.1364/BOE.7.003716
dc.rights.accessRights openAccess
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/333632
dc.relation.projectID Comunidad de Madrid. S2013/ICE-2958/BRADE-CM
dc.relation.projectID Gobierno de España. RYC-2010-05838
dc.relation.projectID Gobierno de España. ISCIII-PI12/01580
dc.type.version publishedVersion
dc.identifier.publicationfirstpage 3716
dc.identifier.publicationissue 9
dc.identifier.publicationlastpage 3720
dc.identifier.publicationtitle Biomedical optics express
dc.identifier.publicationvolume 7
dc.identifier.uxxi AR/0000018051
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