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A Customized Light Sheet Microscope to Measure Spatio-Temporal Protein Dynamics in Small Model Organisms
Autor(es):
Ripoll Lorenzo, Jorge; Rieckher, Matthias; Kyparissidis-Kokkinidis, Ilias; Zacharopoulos, Athanasios; Kourmoulakis, Georgios; Zacharakis, Giannis; Tavernarakis, Nektarios
Fecha de edición:
2015-05-22
Cita:
Rieckher M, Kyparissidis-Kokkinidis I, Zacharopoulos A, Kourmoulakis G, Tavernarakis N, Ripoll J, et al. (2015) A Customized Light Sheet Microscope to Measure Spatio-Temporal Protein Dynamics in Small Model Organisms. PLoS ONE 10(5): e0127869. doi:10.1371/journal.pone.0127869
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0127869
Agradecimientos:
MR, GZ and AZ acknowledge funding from the Projects “Skin-DOCTor” Grant No. 1778 and “Neureka!” Grant No. LSF7-341 implemented under
the "ARISTEIA" and "Supporting Postdoctoral Researchers" Actions respectively, of the "OPERATIONAL PROGRAMME EDUCATION AND
LIFELONG LEARNING", (http://www.espa.gr/en/pages/staticOPEducationandLifelongLearning.aspx),
which is co-funded by the European Social Fund and
National Resources and from the EU Marie Curie Initial Training Network “OILTEBIA”, Grant No. PITNGA-2012-317526 (http://ec.europa.eu/research/mariecurieactions/, http://gdo.uc3m.es/oiltebia/index.
php?option = com_content&view = frontpage). JR acknowledges support from EC FP7 CIG grant HIGHTHROUGHPUT TOMO PICIG12-GA-2012-333632, (http://cordis.europa.eu/projects/333632), and
Spanish MINECO grant MESO-IMAGING FIS2013-41802-R, (https://sede.micinn.gob.es/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the
manuscript.
Versión del editor:
http://dx.doi.org/10.1371/journal.pone.0127869
Proyecto:
info:eu-repo/grantAgreement/EC/FP7/333632
info:eu-repo/grantAgreement/EC/FP7/317526
Gobierno de España. FIS2013-41802-R
info:eu-repo/grantAgreement/EC/FP7/317526
Gobierno de España. FIS2013-41802-R
Derechos:
Atribución 3.0 España
Resumen:
We describe a customizable and cost-effective light sheet microscopy (LSM) platform for rapid three-dimensional imaging of protein dynamics in small model organisms. The system is designed for high acquisition speeds and enables extended time-lapse in vivo exp
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A Customized Light Sheet Microscope to Measure Spatio-Temporal Protein Dynamics in Small Model Organisms
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