A Customized Light Sheet Microscope to Measure Spatio-Temporal Protein Dynamics in Small Model Organisms

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A Customized Light Sheet Microscope to Measure Spatio-Temporal Protein Dynamics in Small Model Organisms

Autor(es): Ripoll Lorenzo, Jorge; Rieckher, Matthias; Kyparissidis-Kokkinidis, Ilias; Zacharopoulos, Athanasios; Kourmoulakis, Georgios; Zacharakis, Giannis; Tavernarakis, Nektarios
Fecha de edición: 2015-05-22
Cita: Rieckher M, Kyparissidis-Kokkinidis I, Zacharopoulos A, Kourmoulakis G, Tavernarakis N, Ripoll J, et al. (2015) A Customized Light Sheet Microscope to Measure Spatio-Temporal Protein Dynamics in Small Model Organisms. PLoS ONE 10(5): e0127869. doi:10.1371/journal.pone.0127869
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0127869
Agradecimientos: MR, GZ and AZ acknowledge funding from the Projects “Skin-DOCTor” Grant No. 1778 and “Neureka!” Grant No. LSF7-341 implemented under the "ARISTEIA" and "Supporting Postdoctoral Researchers" Actions respectively, of the "OPERATIONAL PROGRAMME EDUCATION AND LIFELONG LEARNING", (http://www.espa.gr/en/pages/staticOPEducationandLifelongLearning.aspx), which is co-funded by the European Social Fund and National Resources and from the EU Marie Curie Initial Training Network “OILTEBIA”, Grant No. PITNGA-2012-317526 (http://ec.europa.eu/research/mariecurieactions/, http://gdo.uc3m.es/oiltebia/index. php?option = com_content&view = frontpage). JR acknowledges support from EC FP7 CIG grant HIGHTHROUGHPUT TOMO PICIG12-GA-2012-333632, (http://cordis.europa.eu/projects/333632), and Spanish MINECO grant MESO-IMAGING FIS2013-41802-R, (https://sede.micinn.gob.es/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Versión del editor: http://dx.doi.org/10.1371/journal.pone.0127869
Proyecto: info:eu-repo/grantAgreement/EC/FP7/333632
info:eu-repo/grantAgreement/EC/FP7/317526
Gobierno de España. FIS2013-41802-R
URI: http://hdl.handle.net/10016/20814
Derechos: Atribución 3.0 España
Atribución 3.0 España

Resumen:

We describe a customizable and cost-effective light sheet microscopy (LSM) platform for rapid three-dimensional imaging of protein dynamics in small model organisms. The system is designed for high acquisition speeds and enables extended time-lapse in vivo exp [+]

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