Publication: Combination of Single-Photon Emission Computed Tomography and Magnetic Resonance Imaging to Track ¹¹¹In-Oxine-Labeled Human Mesenchymal Stem Cells in Neuroblastoma-Bearing Mice
Loading...
Identifiers
Publication date
2014-12
Defense date
Advisors
Tutors
Journal Title
Journal ISSN
Volume Title
Publisher
Decker
Impact
Export
Abstract
Homing is an inherent, complex, multistep process performed by cells such as human bone marrow mesenchymal stem cells (hMSCs) to travel from a distant location to inflamed or damaged tissue and tumors. This ability of hMSCs has been exploited as a tumor-targeting strategy in cell-based cancer therapy. The purpose of this study was to investigate the applicability of ¹¹¹In-oxine for tracking hMSCs in vivo by combining single-photon emission computed tomography (SPECT) and magnetic resonance imaging (MRI). ¹¹¹In-labeled hMSCs (10⁶ cells) were infused intraperitoneally in neuroblastoma-bearing mice, whereas a control group received a dose of free ¹¹¹In-oxine. SPECT and MRI studies were performed 24 and 48 hours afterwards. Initially, the images showed similar activity in the abdomen in both controls and hMSC-injected animals. In general, abdominal activity decreases at 48 hours. hMSC-injected animals showed increased uptake in the tumor area at 48 hours, whereas the control group showed a low level of activity at 24 hours, which decreased at 48 hours. In conclusion, tracking ¹¹¹In-labeled hMSCs combining SPECT and MRI is feasible and may be transferable to clinical research. The multimodal combination is essential to ensure appropriate interpretation of the images.
Description
Keywords
Mesenchymal stem cells, Single-photon emission computed tomography, Magnetic resonance imaging
Bibliographic citation
Molecular Imaging, December 2014, 13: 1-10.