Citation:
Scientific Reports 4, article number 7325. doi: 10.1038/srep07325
ISSN:
2045-2322
DOI:
10.1038/srep07325
Sponsor:
This work was supported in part by Project ‘‘THALES – BSRC ‘Alexander Fleming’ –
Development and employment of Minos-based genetic and functional genomic
technologies in model organisms (MINOS)’’ – MIS: 376898, the Fellowship for Young
International Scientist of the Chinese Academy of Sciences Grant No. 2010Y2GA03 and the
NSFC-NIH Biomedical collaborative research program 81261120414. A. Arranz
acknowledges support from Marie Curie Intra-European Fellowship Program
FP7-PEOPLE-2010-IEF. J. Ripoll acknowledges support from EC FP7 CIG grant
HIGH-THROUGHPUT TOMO, and Spanish MINECO grant MESO-IMAGING
FIS2013-41802-R. The authors would like to thank Dr. S. Oehler for the help with the
GFP-expressing flies, and G. Livadaras and G. Zacharakis for help with the Drosophila
stocks.
Rights:
Atribución-NoComercial-CompartirIgual 3.0 España
Abstract:
Even though in vivo imaging approaches have witnessed several new and important developments, specimens that exhibit high light scattering properties such as Drosophila melanogaster pupae are still not easily accessible with current optical imaging techniques,Even though in vivo imaging approaches have witnessed several new and important developments, specimens that exhibit high light scattering properties such as Drosophila melanogaster pupae are still not easily accessible with current optical imaging techniques, obtaining images only from subsurface features. This means that in order to obtain 3D volumetric information these specimens need to be studied either after fixation and a chemical clearing process, through an imaging window - thus perturbing physiological development -, or during early stages of development when the scattering contribution is negligible. In this paper we showcase how Optical Projection Tomography may be used to obtain volumetric images of the head eversion process in vivo in Drosophila melanogaster pupae, both in control and headless mutant specimens. Additionally, we demonstrate the use of Helical Optical Projection Tomography (hOPT) as a tool for high throughput 4D-imaging of several specimens simultaneously.[+][-]