Agradecimientos:
This work was supported in part by the Bill and Melinda Gates
Foundation, the National Basic Research Program of China (973
Program) under Grant No. 2011CB707700, the National Natural
Science Foundation of China under Grant No. 81227901,
81027002, 61231004, and 81101095, the Fellowship for
Young International Scientists of the Chinese Academy of
Sciences under Grant No. 2010Y2GA03, and the Instrument
Developing Project of the Chinese Academy of Sciences
under Grant No. YZ201164. A. Arranz acknowledges support
from the Marie Curie Intra-European Fellowship program
IEF-2010-275137. J.R. acknowledges support from EC FP7
IMI project PREDICT-TB, the EC FP7 CIG grant HIGHTHROUGHPUT
TOMO, and the Spanish MINECO project
grant FIS2013-41802-R MESO-IMAGING.
Proyecto:
info:eu-repo/grantAgreement/EC/FP7/333632 Gobierno de España. FIS2013-41802-R/MESO-IMAGING info:eu-repo/grantAgreement/EC/FP7/IMI-PREDICT-TB info:eu-repo/grantAgreement/EC/FP7/275137
Laser sheet microscopy is a widely used imaging technique for imaging the three-dimensional distribution of a fluorescence signal in fixed tissue or small organisms. In laser sheet microscopy, the stripe artifacts caused by high absorption or high scattering sLaser sheet microscopy is a widely used imaging technique for imaging the three-dimensional distribution of a fluorescence signal in fixed tissue or small organisms. In laser sheet microscopy, the stripe artifacts caused by high absorption or high scattering structures are very common, greatly affecting image quality. To solve this problem, we report here a two-step procedure which consists of continuously acquiring laser sheet images while vertically displacing the sample, and then using the variational stationary noise remover (VSNR) method to further reduce the remaining stripes. Images from a cleared murine colon acquired with a vertical scan are compared with common stitching procedures demonstrating that vertically scanned light sheet microscopy greatly improves the performance of current light sheet microscopy approaches without the need for complex changes to the imaging setup and allows imaging of elongated samples, extending the field of view in the vertical direction.[+][-]
