Cita:
Optics express: the international online journal of optics, vol. 22 issue 19 (Sept., 2014)
ISSN:
1094-4087
DOI:
10.1364/OE.22.023215
Agradecimientos:
This work was supported by the Spanish MINECO grant DPI2012-32994. JR acknowledges
support from Marie Curie CIG “High-Throughput Tomo” and Spanish MINECO grant
FIS2013-41802-R.
A new method to obtain the three-dimensional localization of fluorochrome distributions in micrometric samples is presented. It uses a microlens array coupled to the image port of a standard microscope to obtain tomographic data by a filtered back-projection aA new method to obtain the three-dimensional localization of fluorochrome distributions in micrometric samples is presented. It uses a microlens array coupled to the image port of a standard microscope to obtain tomographic data by a filtered back-projection algorithm. Scanning of the microlens array is proposed to obtain a dense data set for reconstruction. Simulation and experimental results are shown and the implications of this approach in fast 3D imaging are discussed.[+][-]