Helical Optical Projection Tomography

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dc.contributor.author Arranz, Alicia
dc.contributor.author Dong, Di
dc.contributor.author Zhu, Shouping
dc.contributor.author Rudin, Markus
dc.contributor.author Tsatsanis, Christos
dc.contributor.author Tian, Jie
dc.contributor.author Ripoll Lorenzo, Jorge
dc.date.accessioned 2013-10-29T17:31:12Z
dc.date.available 2013-10-29T17:31:12Z
dc.date.issued 2013-11-04
dc.identifier.bibliographicCitation Optics Express, (2013), 21(22), 25912-25925.
dc.identifier.issn 1094-4087
dc.identifier.uri http://hdl.handle.net/10016/17857
dc.description.abstract A new technique termed Helical Optical Projection Tomography (hOPT) has been developed with the aim to overcome some of the limitations of current 3D optical imaging techniques. hOPT is based on Optical Projection Tomography (OPT) with the major difference that there is a translation of the sample in the vertical direction during the image acquisition process, requiring a new approach to image reconstruction. Contrary to OPT, hOPT makes possible to obtain 3D-optical images of intact long samples without imposing limits on the sample length. This has been tested using hOPT to image long murine tissue samples such as spinal cords and large intestines. Moreover, 3D-reconstructed images of the colon of DSS-treated mice, a model for Inflammatory Bowel Disease, allowed the identification of the structural alterations. Finally, the geometry of the hOPT device facilitates the addition of a Selective Plane Illumination Microscopy (SPIM) arm, providing the possibility of delivering high resolution images of selected areas together with complete volumetric information
dc.description.sponsorship This work was partially supported by EC FP7 collaborative grant FMT-XCT and the Bill and Melinda Gates foundation. A.A. wishes to acknowledge support from Marie Curie IEF-2010-275137. J.R. wishes to acknowledge support from EC FP7 IMI project PREDICT-TB, and the EC FP7 CIG grant HIGH-THROUGHPUT TOMO. D.D., S.Z. and J.T. acknowledge support from the National Basic Research Program of China (973 Program) under Grant 2011CB707700, the Fellowship for Young International Scientists of the Chinese Academy of Sciences under Grant 2010Y2GA03, the National Natural Science Foundation of China under Grant 81101084 and Instrument Developing Project of the Chinese Academy of Sciences under Grant No. YZ201164
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Optical Society of America
dc.subject.other Fluorescence microscopy
dc.subject.other Three-dimensional microscopy
dc.subject.other Image reconstruction techniques
dc.subject.other Light propagation in tissues
dc.subject.other Tomography
dc.title Helical Optical Projection Tomography
dc.type article
dc.description.status Publicado
dc.relation.publisherversion http://dx.doi.og/10.1364/OE.21.025912
dc.subject.eciencia Biología y Biomedicina
dc.identifier.doi 10.1364/OE.21.025912
dc.rights.accessRights openAccess
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/275137
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/333632
dc.type.version publishedVersion
dc.identifier.publicationfirstpage 25912
dc.identifier.publicationissue 22
dc.identifier.publicationlastpage 25925
dc.identifier.publicationtitle Optics Express
dc.identifier.publicationvolume 21
dc.identifier.uxxi AR/0000013953
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