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Atribución-NoComercial-SinDerivadas 3.0 España
Abstract:
This work describes a systematic evaluation of several
autofocus functions used for analytical fluorescent image
cytometry studies of counterstained nuclei. Focusing is the
first step in the automatic fluorescence in situ hybridization
analysis of cells. TThis work describes a systematic evaluation of several
autofocus functions used for analytical fluorescent image
cytometry studies of counterstained nuclei. Focusing is the
first step in the automatic fluorescence in situ hybridization
analysis of cells. Thirteen functions have been evaluated
using qualitative and quantitative procedures. For the last
of these procedures a figure-of-merit (FOM) is defined and
proposed. This new FOM takes into account five important
features of the focusing function. Our results show that
functions based on correlation measures have the best
performance for this type of image.[+][-]