Agradecimientos:
This work was supported by grants from the Ministerio de Ciencia y Tecnología (TEC2004-07052), Ministerio de Sanidad y Consumo (CIBERsam CB07/09/0031 and Plan Nacional sobre Drogas 2007/043), Ministerio de Industria (CDTEAM Project), and Fundación de Investigación Médica Mutua Madrileña. We thank Alexandra de Francisco for her assistance with the PET studies, the Atomic, Molecular, and Nuclear Physics Department of the Universidad Complutense in Madrid for reconstructing the PET images, and the National Institute of Health for facilitating the piPET system.
Purpose: This study was designed to assess changes in brain glucose metabolism in rats after
visual stimulation.
Materials and methods: We sought to determine whether visual activation in the rat brain could
be detected using a small-animal positron emissioPurpose: This study was designed to assess changes in brain glucose metabolism in rats after
visual stimulation.
Materials and methods: We sought to determine whether visual activation in the rat brain could
be detected using a small-animal positron emission tomography (PET) scanner and 2-deoxy-2-
[18F]fluoro-D-glucose (FDG). Eleven rats were divided into two groups: (a) five animals exposed
to ambient light and (b) six animals stimulated by stroboscopic light (10 Hz) with one eye
covered. Rats were injected with FDG and, after 45 min of visual stimulation, were sacrificed and
scanned for 90 min in a dedicated PET tomograph. Images were reconstructed by a threedimensional
ordered subset expectation maximization algorithm (1.8 mm full width at half
maximum). A region-of-interest (ROI) analysis was performed on 14 brain structures drawn on
coronal sections. Statistical parametric mapping (SPM) adapted for small animals was also
carried out. Additionally, the brains of three rats were sliced into 20-μm sections for
autoradiography.
Results: Analysis of ROI data revealed significant differences between groups in the right
superior colliculus, right thalamus, and brainstem (p≤0.05). SPM detected the same areas as
the ROI approach. Autoradiographs confirmed the existence of hyperactivation in the left
superior colliculus and auditory cortex.
Conclusions: To our knowledge, this is the first report that uses FDG-PET and SPM analysis to show changes in rat brain glucose metabolism after a visual stimulus[+][-]
