Publication:
Biomedical applications of tissue clearing and three-dimensional imaging in health and disease

Loading...
Thumbnail Image
Identifiers
Publication date
2020-08-21
Defense date
Advisors
Tutors
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Impact
Google Scholar
Export
Research Projects
Organizational Units
Journal Issue
Abstract
Three-dimensional (3D) optical imaging techniques can expand our knowledge about physiological and pathological processes that cannot be fully understood with 2D approaches. Standard diagnostic tests frequently are not sufficient to unequivocally determine the presence of a pathological condition. Whole-organ optical imaging requires tissue transparency, which can be achieved by using tissue clearing procedures enabling deeper image acquisition and therefore making possible the analysis of large-scale biological tissue samples. Here, we review currently available clearing agents, methods, and their application in imaging of physiological or pathological conditions in different animal and human organs. We also compare different optical tissue clearing methods discussing their advantages and disadvantages and review the use of different 3D imaging techniques for the visualization and image acquisition of cleared tissues. The use of optical tissue clearing resources for large-scale biological tissues 3D imaging paves the way for future applications in translational and clinical research.
Description
Keywords
Biomedical discipline, Imaging methods in chemistry, Medical imaging, Optical imaging
Bibliographic citation
Gómez-Gaviro, M. V., Sanderson, D., Ripoll, J., & Desco, M. (2020). Biomedical applications of tissue clearing and Three-Dimensional Imaging in health and disease. iScience, 23(8), 101432.