Differentiation of human umbilical vein endothelial cells (HUVECS) into tubular structures resembling immature capillaries

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Angiogenesis is the mechanism of new vessel formation from preexisting ones. It is a process of major relevance in tissue development and, more prominently, in pathological conditions. Kindler Syndrome, a rare disease affecting protein kindlin1, disrupts the dermo-epidermal junction resulting in skin blistering.! ! In order to interrogate the angiogenic effect of the Kindler Syndrome, bovine aortic endothelial cells and human umbilical vein endothelial cells were cultured in fibrin gel and MatrigelTM basement matrix. Qualitative analysis of the former indicated an enhanced angiogenic activity at basement membrane level induced by the Kindler genotype in human keratinocytes. This result was confirmed a posteriori by quantitative analysis with the Angiogenesis Analyzer plugin of ImageJ.! ! The quantitative results point to an increased release of mitogenic substances by keratinocytes lining the epidermis. This has the effect of enhancing angiogenesis in the dermal layer of the skin and is evidenced by the increased number of branches and branch length found under Kindler conditions, indicative of sprouting angiogenesis. Increased mesh area and number, along with a reduced mean mesh size found under Kindler conditions are indicative of an enhanced intussuspective angiogenic activity. The analysis of these five key parameters strongly suggest the proangiogenic influence of the Kindler genotype, expressed in epidermal keratinocytes, which affects vessel formation in the dermal layer of the skin.
Biología celular, Histología, Angiogénesis, Vasos sanguíneos, Cell biology, Angiogenesis, Endothelial Cells, Blood vessels
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