Publication: Assessment of airway distribution of transnasal solutions in mice by PET/CT imaging
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ISSN: 1536-1632 (print version)
ISSN: 1860-2002 (electronic version)
Publication date
2009
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Tutors
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Publisher
Springer
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Abstract
Transnasal administration is one of the most common routes for allergen challenge in mouse models of airway diseases. Although this technique is widely used, neither the amount of allergen that reaches the lung nor its airway distribution has been well established. We used positron emission tomography (PET) and computed tomography (CT) to examine the anatomical
distribution of a solution containing a tracer immediately after transnasal delivery and to determine the possible influence of age and administered volume.
Procedures: Forty-six female BALB/c mice were divided into three groups according to
instillation volume and age: (A) 15 μl, 8–10 weeks old (N=10), (B) 30 μl, 8–10 weeks old (N=20), and (C) 30 μl, 32 weeks old (N=16). Anesthetized animals underwent a dynamic scan in a dedicated small-animal PET scanner immediately after transnasal administration of a solution containing 18FDG. Regions of interest were used to obtain quantitative data. Animals were also
imaged with a small-animal CT scanner to obtain complementary anatomical information.
Results: Mean±SD (5.69±4.51%) of the solution administered reached the lungs in group A,41.84±8.03% in group B, and 36.65±16.15% in group C. A comparable percentage was delivered to the left and right lungs in all the groups. Analysis of variance revealed a significant difference between the groups in the proportion of the solution that reached the lungs depending
on the injection volume (PG0.001), but not depending on animal age.
Conclusions: In this first report on quantitative imaging by PET and CT in small animals, we confirmed the suitability of the transnasal route with an instilled volume of 30 μl delivering fluids into the lower airways, although only about 40% of the dose reaches the lungs
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Keywords
PET, FDG, CT, Transnasal administration, Bronchial asthma
Bibliographic citation
Molecular Imaging and Biology, 2009, vol. 11, n. 4, p. 263-268