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Functional screening of selective mitochondrial inhibitors of Plasmodium

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dc.affiliation.dptoUC3M. Departamento de Bioingenieríaes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: BSEL - Laboratorio de Ciencia e Ingeniería Biomédicaes
dc.contributor.authorGómez Lorenzo, María G.
dc.contributor.authorRodríguez Alejandre, Ane
dc.contributor.authorMoliner Cubel, Sonia
dc.contributor.authorMartínez Hoyos, María
dc.contributor.authorBahamontes Rosa, Noemí
dc.contributor.authorGonzález Del Río, Rubén
dc.contributor.authorRódenas, Carolina
dc.contributor.authorDe La Fuente, Jesús
dc.contributor.authorLavandera, José Luis
dc.contributor.authorGarcía Bustos, José F.
dc.contributor.authorMendoza Losana, Alfonso
dc.date.accessioned2024-01-26T11:19:29Z
dc.date.available2024-01-26T11:19:29Z
dc.date.issued2018-05-15
dc.description.abstractPhenotypic screening has produced most of the new chemical entities currently in clinical development for malaria, plus many lead compounds active against Plasmodium falciparum asexual stages. However, lack of knowledge about the mode of action of these compounds delays and may even hamper their future development. Identifying the mode of action of the inhibitors greatly helps to prioritise compounds for further development as novel antimalarials. Here we describe a whole-cell method to detect inhibitors of the mitochondrial electron transport chain, using oxygen consumption as high throughput readout in 384-well plate format. The usefulness of the method has been confirmed with the Tres Cantos Antimalarial Compound Set (TCAMS). The assay identified 124 respiratory inhibitors in TCAMS, seven of which were novel anti-plasmodial chemical structures never before described as mitochondrial inhibitors.es
dc.format.extent9es
dc.format.mimetypeapplication/pdfen
dc.identifier.bibliographicCitationGomez-Lorenzo, M. G., Rodríguez-Alejandre, A., Moliner-Cubel, S., Martínez-Hoyos, M., Bahamontes-Rosa, N., Gonzalez del Rio, R., Ródenas, C., Fuente, J. de la, Lavandera, J. L., García-Bustos, J. F., & Mendoza-Losana, A. (2018). Functional screening of selective mitochondrial inhibitors of Plasmodium. International Journal for Parasitology: Drugs and Drug Resistance 8(2), pp. 295-303)es
dc.identifier.doihttps://doi.org/10.1016/j.ijpddr.2018.04.007
dc.identifier.issn2211-3207
dc.identifier.publicationfirstpage295es
dc.identifier.publicationissue2es
dc.identifier.publicationlastpage303es
dc.identifier.publicationtitleInternational Journal for Parasitology-Drugs and Drug Resistancees
dc.identifier.publicationvolume8es
dc.identifier.urihttps://hdl.handle.net/10016/39559
dc.identifier.uxxiAR/0000030691
dc.language.isoenges
dc.publisherElsevier BVes
dc.rights© 2018 The Authorses
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes
dc.rights.accessRightsopen accessen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/es/deed.es*
dc.subject.otherPlasmodium falciparumen
dc.subject.otherPlasmodium Yoeliien
dc.subject.otherOxygen consumptionen
dc.subject.otherMitochondrial inhibitors and cytochromeen
dc.titleFunctional screening of selective mitochondrial inhibitors of Plasmodiumen
dc.typeresearch articleen
dc.type.hasVersionVoRen
dspace.entity.typePublication
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