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Formation and maintenance of nitrogen-fixing cell patterns in filamentous cyanobacteria

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dc.affiliation.dptoUC3M. Departamento de Matemáticases
dc.affiliation.grupoinvUC3M. Grupo de Investigación: Interdisciplinar de Sistemas Complejos (GISC)es
dc.contributor.authorMuñoz-García, Javier
dc.contributor.authorAres García, Saúl
dc.date.accessioned2016-06-10T12:16:50Z
dc.date.available2016-06-10T12:16:50Z
dc.date.issued2016-04es
dc.description.abstractCyanobacteria forming one-dimensional filaments are paradigmaticmodel organisms of the transition between unicellular andmulticellular living forms. Under nitrogen-limiting conditions, infilaments of the genus Anabaena, some cells differentiate into heterocysts,which lose the possibility to divide but are able to fixenvironmental nitrogen for the colony. These heterocysts form aquasiregular pattern in the filament, representing a prototype ofpatterning and morphogenesis in prokaryotes. Recent years haveseen advances in the identification of the molecular mechanism regulatingthis pattern. We use these data to build a theory on heterocystpattern formation, for which both genetic regulation and theeffects of cell division and filament growth are key components. Thetheory is based on the interplay of three generic mechanisms: localautoactivation, early long-range inhibition, and late long-range inhibition.These mechanisms can be identified with the dynamics ofhetR, patS, and hetN expression. Our theory reproduces quantitativelythe experimental dynamics of pattern formation and maintenancefor wild type and mutants. We find that hetN alone is notenough to play the role as the late inhibitory mechanism: a secondmechanism, hypothetically the products of nitrogen fixation suppliedby heterocysts, must also play a role in late long-range inhibition.The preponderance of even intervals between heterocysts arisesnaturally as a result of the interplay between the timescales of geneticregulation and cell division.We also find that a purely stochasticinitiation of the pattern, without a two-stage process, is enoughto reproduce experimental observations.en
dc.description.sponsorshipFunding from the Spanish Ministry of Economy and Competitiveness through Grant PHYSDEV (FIS2012-32349) and the Ramón y Cajal Program (to S.A.).en
dc.format.extent6es
dc.format.mimetypeapplication/pdf
dc.identifier.bibliographicCitationProceedings of the National Academy of Sciences of the United States of America (PNAS), v. 113, n. 22, April 2016, pp. 6218-6223en
dc.identifier.doihttps://doi.org/10.1073/pnas.1524383113es
dc.identifier.issn1091-6490es
dc.identifier.publicationfirstpage6218
dc.identifier.publicationissue22
dc.identifier.publicationlastpage6223
dc.identifier.publicationtitleProceedings of the National Academy of Sciencesen
dc.identifier.publicationvolume113
dc.identifier.urihttps://hdl.handle.net/10016/23157
dc.identifier.uxxiAR/0000017979
dc.language.isoeng
dc.publisherPNAS
dc.relation.projectIDGobierno de España. FIS2012-32349/PHYSDEV
dc.relation.publisherversionhttp://dx.doi.org/10.1073/pnas.1524383113
dc.rightsProceedings of the National Academy of Sciences of the United States of America (PNAS)en
dc.rights.accessRightsopen access
dc.subject.ecienciaBiología y Biomedicinaes
dc.subject.otherCyanobacteriaen
dc.subject.otherPattern formationen
dc.subject.otherActivator–inhibitoren
dc.subject.otherHeterocyst differentiationen
dc.subject.otherGene-regulatory networksen
dc.titleFormation and maintenance of nitrogen-fixing cell patterns in filamentous cyanobacteriaen
dc.typeresearch article*
dc.type.hasVersionVoR*
dspace.entity.typePublication
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