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3D imaging in CUBIC-cleared mouse heart tissue: going deeper

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dc.affiliation.dptoUC3M. Departamento de Bioingenieríaes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: Biomedical Imaging and Instrumentation Groupes
dc.affiliation.grupoinvUC3M. Grupo de Investigación: BSEL - Laboratorio de Ciencia e Ingeniería Biomédicaes
dc.contributor.authorNehrhoff, Imke
dc.contributor.authorBocancea, Diana
dc.contributor.authorVaquero, Javier
dc.contributor.authorVaquero López, Juan José
dc.contributor.authorRipoll Lorenzo, Jorge
dc.contributor.authorDesco Menéndez, Manuel
dc.contributor.authorGómez Gaviro, María Victoria
dc.date.accessioned2020-05-05T13:22:25Z
dc.date.available2020-05-05T13:22:25Z
dc.date.issued2016-08
dc.description.abstractThe ability to acquire high resolution 3D images of the heart enables to study heart diseases more in detail. In this work, the CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) clearing protocol was optimized for thick mouse heart sections to enhance the penetration depth of the confocal microscope lasers into the tissue. In addition, the optimized CUBIC clearing of the heart enhances antibody penetration into the tissue by a factor of five. The present protocol enables deep 3D high-quality image acquisition in the heart allowing a much more accurate assessment of the cellular and structural changes that underlie heart diseases.en
dc.description.sponsorshipHuman Frontiers Scientific Program (RGP0004/2013); EC FP7 CIG grant HIGH THROUGHPUT TOMO; Comunidad de Madrid BRADE (S2013/ICE-2958); MINECO. Ramón y Cajal program (RYC 2010-05838); MINECO, ISCIII-Fondos FEDER (PI12/01580).
dc.description.sponsorshipEuropean Community's Seventh Framework Programen
dc.format.mimetypeapplication/pdf
dc.identifier.bibliographicCitationBiomedical Optics Express, vol. 7, n. 9, Sept. 2016, 3716-3720
dc.identifier.doihttps://doi.org/10.1364/BOE.7.003716
dc.identifier.issn2156-7085
dc.identifier.publicationfirstpage3716
dc.identifier.publicationissue9
dc.identifier.publicationlastpage3720
dc.identifier.publicationtitleBiomedical optics expressen
dc.identifier.publicationvolume7
dc.identifier.urihttps://hdl.handle.net/10016/23519
dc.identifier.uxxiAR/0000018051
dc.language.isoeng
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/333632
dc.relation.projectIDComunidad de Madrid. S2013/ICE-2958/BRADE-CM
dc.relation.projectIDGobierno de España. RYC-2010-05838
dc.relation.projectIDGobierno de España. ISCIII-PI12/01580
dc.rights© 2016 Optical Society of Americaen
dc.rights.accessRightsopen access
dc.subject.ecienciaBiología y Biomedicinaes
dc.title3D imaging in CUBIC-cleared mouse heart tissue: going deeperen
dc.typeresearch article*
dc.type.hasVersionVoR*
dspace.entity.typePublication
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